Key Documents
AP1035
Anti-Cyclophilin D Mouse mAb (E11AE12BD4)
Synonym(s):
Anti-CyPD
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About This Item
antibody form
purified antibody
clone
E11AE12BD4, monoclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity
mouse, human, bovine, rat
isotype
IgG1
General description
Cyclophilin D is a member of the immunophilin family of soluble cytosolic receptors capable of binding to one of two major immunosuppressant agents, cyclosporin A (CsA) or FK506. Proteins that bind CsA are called cyclophilins. Immunophilins function as peptidyl prolyl cis-trans-isomerases (PPlase) whose activity is inhibited by the respective immunosuppressant compounds. Immunophilins accelerate folding of proteins in vivo and in vitro by catalyzing slow steps in the initial folding and rearrangement of proline-containing proteins. Cyclophilin D is located in the intermembrane space of the mitochondria where it is an integral member of the permeability transition pore complex that includes the voltage dependent anion channel (VDAC) and adenine nucleotide translocase (ANT).
Mouse monoclonal antibody purified by ammonium sulfate precipitation. Recognizes the ~17-18 kDa processed form of mitochondrial cyclophilin D (CyPD) protein.
Recognizes the ~17-18 kDa processed form of mitochondrial cyclophilin D in cultured fibroblasts.
This Anti-Cyclophilin D Mouse mAb (E11AE12BD4) is validated for use in Immunoblotting, Immunocytochemistry for the detection of Cyclophilin D.
Immunogen
recombinant, rat cyclophilin D
Application
Immunoblotting (1 μg/ml)
Immunocytochemistry (5 μg/ml)
Immunocytochemistry (5 μg/ml)
Physical form
In HBS.
Preparation Note
Following initial thaw, aliquot and freeze (-20°C).
Analysis Note
Positive Control
Heart mitochondria
Heart mitochondria
Other Notes
Useful for detection of apoptosis related proteins. Antibody should be titrated for optimal results in individual systems.
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Andrew P Halestrap et al.
Current medicinal chemistry, 10(16), 1507-1525 (2003-07-23)
In addition to its normal function, the adenine nucleotide translocase (ANT) forms the inner membrane channel of the mitochondrial permeability transition pore (MPTP). Binding of cyclophilin-D (CyP-D) to its matrix surface (probably on Pro(61) on loop 1) facilitates a calcium-triggered
Gina Sánchez et al.
PloS one, 15(5), e0233591-e0233591 (2020-05-27)
The heart is critically dependent on mitochondrial respiration for energy supply. Ischemia decreases oxygen availability, with catastrophic consequences for cellular energy systems. After a few minutes of ischemia, the mitochondrial respiratory chain halts, ATP levels drop and ion gradients across
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