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Key Documents

CA1004

Millipore

Anti-CA 125 Mouse mAb (OC 125)

liquid, clone OC 125, Calbiochem®

Synonym(s):

Anti-Cancer Antigen 125

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About This Item

UNSPSC Code:
12352203

biological source

mouse

antibody product type

primary antibodies

clone

OC 125, monoclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

human

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

isotype

IgG1

shipped in

wet ice

storage temp.

2-8°C

General description

This product has been discontinued.



Recognizes the CA125 antigen on the >200 kDa mucin-like glycoprotein in non-mucinous epithelial ovarian carcinoma or uterine myoma.

Anti-CA 125, mouse monoclonal, clone OC125, recognizes the CA125 in non-mucinous epithelial ovarian carcinoma and uterine myoma. It is validated for WB, ICC, IP, and for frozen and paraffin sections.
Purified mouse monoclonal antibody. Recognizes the CA125 antigen on the >200 kDa mucin-like glycoprotein.

Immunogen

Human
ovarian cancer cell line OVCA433

Application


Frozen Sections (see application references)
Immunoblotting (see application references)
Immunoprecipitation (see application references)
Immunocytochemistry (see application references)
Paraffin Sections (1:40, heat pre-treatment required, see comments)

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In 10 mM PBS, 1% BSA.

Preparation Note

Following initial use, aliquot and freeze (-20°C) for long-term storage if desired. Avoid freeze/thaw.

Analysis Note

Negative Control
Normal mouse serum
Positive Control
Non-mucinous epithelial ovarian carcinoma, serous and endometroid adenocarcinomas, Krukenberg′s tumor, or uterine myoma

Other Notes

Hogdall, E.V., et al. 2002. APMIS110, 545.
Deng, X., et al. 2000. Gynecol. Oncol.79, 416.
Jeyarajah, A.R., et al. 1999. Gynecol. Oncol.73, 242.
Works for formalin-fixed paraffin-embedded tissues. Pretreat with an antigen unmasking solution and heat for best results. Primary antibody may be diluted 1:40 for biotin based detection systems. For optimal staining, the primary antibody should be incubated 20-60 min at room temperature. Antibody should be titrated for optimal results in individual systems.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Highly Toxic (H)

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Storage Class Code

10-13 - German Storage Class 10 to 13

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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