Anti-S100B Mouse mAb (1B2)

Purified mouse monoclonal antibody. Recognizes the ~12 kDa S100B protein.

Recognizes the ~10 kDa S100B protein in HepG2 cells and human salivary gland tissue.

The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21; however, this gene is located at 21q22.3. This protein may function in Neurite extension, proliferation of melanoma cells, stimulation of Ca²⁺ fluxes, inhibition of PKC-mediated phosphorylation, astrocytosis and axonal proliferation, and inhibition of microtubule assembly. Chromosomal rearrangements and altered expression of this gene have been implicated in several neurological, neoplastic, and other types of diseases, including Alzheimer′s disease, Down′s syndrome, epilepsy, amyotrophic lateral sclerosis, melanoma, and type I diabetes.

This Anti-S100B Mouse mAb (1B2) is validated for use in Immunoblotting, Paraffin Sections for the detection of S100B.

A recombinant polypeptide corresponding to amino acids of 1-93 human S100B, expressed as a GST fusion protein

Immunoblotting (1-5 μg/ml)
Paraffin Sections (3 μg/ml)

In PBS, pH 7.2.

Following initial thaw, aliquot and freeze (-20°C).

Positive Control
HepG2 cells, Human salivary gland tissue

Variables associated with assay conditions will dictate the proper working dilution.