usage
sufficient for 72 tests
Quality Level
packaging
pkg of 1 96-well plate(s)
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
avoid repeated freeze/thaw cycles
protect from light
input
sample type adherent cultured cells
fluorescence
λex ~485 nm
λem ~520 nm
detection method
fluorometric
shipped in
wet ice
General description
A convenient assay for the determination of the relative attachment of adherent cell lines to fibronectin. Cells are seeded onto the fibronectin plates followed by determination of relative cell attachment using a fluorescent dye.
Biochem/physiol Actions
The Innocyte Cell Adhesion Microplate Assays are designed for the determination of the relative attachment of adherent cell lines to extracellular matrix proteins such as Human Fibronectin (Cat. No. CBA011), Human Vitronectin (Cat. No. CBA012), and Human Collagen IV (Cat. No. CBA013). Cells are seeded onto a coated substrate, which is followed by the determination of relative cell attachment using a fluorescent dye.
Features and Benefits
Assay Time: 2.5 h
Preparation Note
• Calcein-AM Working Solution: Warm the calcein-AM solution and the D-PBS to room temperature. Dilute the calcein-AM stock solution 1:300 with D-PBS, i.e. add 20 µl (calcein-AM solution) to 6 ml of D-PBS. This calcein-AM working solution cannot be stored.
Upon arrival store the Calcein-AM at -20°C and the remaining components at 4°C.
Other Notes
Coated 96-Well Plate, Fluorescent Dye, D-PBS, and a user protocol.
Hynes, R.O., et al. 1999. Proc. Natl. Acad. Sci. USA 96, 2588.
Ohashi, T., et al. 1999. Proc. Natl. Acad. Sci USA 96, 2153.
Grande, J.P., et al. 1997. J. Lab. Clin. Med. 130, 476.
Nelson, P.R., et al. 1997. Vasc. Surg. 26, 104.
Horowitz, A.F., et al. 1996. Trends Cell Biol. 6, 460.
Hynes, R.O., et al. 1990. Fibronectins, Springer-Verlag, New York.
Preissner, K.T., et al. 1990. J. Biol. Chem. 265, 18490.
Ohashi, T., et al. 1999. Proc. Natl. Acad. Sci USA 96, 2153.
Grande, J.P., et al. 1997. J. Lab. Clin. Med. 130, 476.
Nelson, P.R., et al. 1997. Vasc. Surg. 26, 104.
Horowitz, A.F., et al. 1996. Trends Cell Biol. 6, 460.
Hynes, R.O., et al. 1990. Fibronectins, Springer-Verlag, New York.
Preissner, K.T., et al. 1990. J. Biol. Chem. 265, 18490.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Multiple Toxicity Values, refer to MSDS (O)
Storage Class Code
10-13 - German Storage Class 10 to 13
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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