Anti-EBNA Mouse mAb (E8.26)

Anti-EBNA, mouse monoclonal, clone E8.26, recognizes the ~88 kDa wild-type EBNA from EBV-transformed human cells and the ~31.5 kDa mutant EBNA in Raji cells. It is validated for use in WB, IF & IP.

Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with P3 x 63Ag 8.653 mouse myeloma cells (see application references). Recognizes the ~88 kDa wild-type and the ~31.5 kDa mutant EBNA proteins.

Recognizes the ~88 kDa wild-type EBNA from EBV-transformed human cells as well as the ~31.5 kDa mutant form of EBNA in Raji cells.

EBNA protein

Immunoblotting (1-5 µg/ml)

Immunofluorescence (1-5 µg/ml)

Immunoprecipitation (1-5 µg/sample)

Lyophilized from 20 mM ammoinum bicarbonate, 100µg BSA.

Reconstitute the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml; product will be more stable if 0.1% sodium azide is included (do not add azide if antibody is to be used with viable cells). Lyophilized antibody should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Following reconstitution, refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles of solutions.

Negative Control
HL60 cells

Positive Control
Raji cells

Antibody should be titrated for optimal results in individual systems.

Freese, U.K., et al. 1983. J. Virol.48, 731.
Fischer, D.K., et al. 1981. Cell24, 543.
Orellana, T., et al. 1977. J. Virol.22, 321.
Lindahl, T., et al. 1976 J. Mol. Biol.102, 511.
Henle, W., et al. 1967 Science157, 1064.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Toxicity: Standard Handling (A)