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FCMAB249A4

Sigma-Aldrich

Milli-Mark® Anti-phospho-PLCγ-1 (Tyr783)-Alexa Fluor™ 488 Antibody, clone 12F4.2

clone 12F4.2, Milli-Mark®, from mouse

Synonym(s):

phospholipase C, gamma 1, 1-phosphatidyl-D-myo-inositol-4,5-bisphosphate, monophosphatidylinositol phosphodiesterase, triphosphoinositide phosphodiesterase, phosphatidylinositol phospholipase C, inositoltrisphosphohydrolase, phospholipase C, gamma 1 (for

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

conjugate

ALEXA FLUOR™ 488

antibody product type

primary antibodies

clone

12F4.2, monoclonal

species reactivity

human

manufacturer/tradename

Milli-Mark®

technique(s)

flow cytometry: suitable

isotype

IgG1

NCBI accession no.

NP_002651.2

UniProt accession no.

P19174

shipped in

wet ice

General description

148 kDa Calculated
Phospholipase C (PLC) is a group of enzymes that play a critical role in signal transduction during the hydrolysis of membrane phospholipids as a response to extracellular stimuli. Part of the phosphinositide-specific PLC family, PLC У-1 is known for its involvement in growth-factor-dependent signal transduction. PLC У-1 is made up of 2 SH2 domains, one SH3 domain and 2 tyrosine phophrylation sites. Almost all growth factors can stimulate tyrosine phosphorylation and subsequent activation of PLC У-1. (Tvorogov, 2005). Of the protein kinases that stimulate tyrosine phosphorylation, PLC У-1 is mostly dependent on three different kinds; ZAP-10, Lck, and Itk. (Kang, 2003). Activation of PLC У-1 catalyzes the hydrolysis of phosphatidylinositol 4,5-biphosphate which in turn produces two second messengers, diacylglycerol (DAG) and inositol 1,4,5-triphosphate(IP3). DAG serves to activate protein Kinase 3, while IP3 mediates the release of calcium. PLC У-1 is crucial in its role to the mediation of cellular proliferation and differentiation.

Application

Detect phospho-PLCγ-1 (Tyr783) using this Milli-Mark Anti-phospho-PLCγ-1 (Tyr783)-Alexa Fluor 488 Antibody, clone 12F4.2 validated for use in FC.

Analysis Note

Evaluated by flow cytometry using A431 cells.

Legal Information

ALEXA FLUOR is a trademark of Life Technologies
MILLI-MARK is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

10-13 - German Storage Class 10 to 13

Certificates of Analysis (COA)

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