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GR31L

Sigma-Aldrich

Anti-IGF-IR (Ab-3) Mouse mAb (33255.111)

lyophilized, clone 33255.111, Calbiochem®

Synonym(s):

Anti-IGF-R, Anti-Insulin-Like Growth Factor Receptor, Anti-Insulin-Like Growth Factor Receptor, Anti-IGF-R

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About This Item

UNSPSC Code:
12352203

biological source

mouse

Quality Level

100

antibody product type

primary antibodies

clone

33255.111, monoclonal

form

lyophilized

does not contain

preservative

species reactivity

human

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

isotype

IgG1

shipped in

ambient

storage temp.

−20°C

General description

This product has been discontinued.



Recognizes the ~250-260 kDa IGF-1 receptor protein in MCF-7 cells. Does not cross-react with recombinant human IGF-I binding protein 1, IGF-I, or IGF-II.

Protein G purified mouse monoclonal antibody. Recognizes the ~250-260 kDa IGF-1 receptor protein.

Immunogen

Epitope: Extracellular domain of IGF-I Receptor
recombinant, human, soluble IGF-I receptor

Application

ELISA (2 µg/ml, capture antibody)

Immunoblotting (non-denaturing protocols only)

Neutralization Studies (11 µg/ml, see comments)

Physical form

Lyophilized from PBS, 20 mM ammonium bicarbonate, ≤5% trehalose, 100 µg BSA.

Preparation Note

Reconstitute with sterile PBS to the desired concentration. Following reconstitution, antibody can be stored at 4°C for up to 1 month. For long-term storage, aliquot and freeze (-20°C).

Analysis Note

Positive Control
Recombinant IGF-I receptor protein or MCF7 cells

Other Notes

Endotoxins: ≤1 EU/µg anti-IGF-IR. This antibody was selected for its ability to block cell surface IGF-I receptor mediated IGF-I and IGF-II bioactivities. The exact concentration of antibody required to neutralize the human cell surface IGF-I receptor mediated bioactivity is dependent on the IGF concentration as well as on the number and types of IGF receptors present on the cell surface (as a function of cell type and culture conditions). A concentration of 11 µg/ml neutralizes ~50-75% of IGF-I receptor-mediated rhIGF-I biological activity in MCF7 cells. This antibody recognizes a conformational epitope. The detection limit for human recombinant IGF-I receptor by immunoblotting is approximately 25 ng/lane. The detection limit for human recombinant IGF-I receptor is ~0.16 ng/well. Based on immunoblots and ELISA, this antibody does not cross-react with human recombinant IGF-I bp1, human recombinant IGF-I and human recombinant IGF-II. Antibody should be titrated for optimal results in individual systems.
Leroith, D., and Roberts, C. T., 2003. Cancer Letter195, 127.
Dore, S., et al. 1997. Trends Neurosci.20, 326.
Dore, S., et al. 1997. Proc. Natl. Acad. Sci. USA94, 4772.
Walter, H.J., et al. 1997. Endocrinology138, 3024.
Beilharz, E.J., et al. 1995. Mol. Brain Res.29, 81.
Jones, J.I. and Clemmons, D.R. 1995. Endocr. Rev.16, 3.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Harmful (C)

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Storage Class Code

10-13 - German Storage Class 10 to 13

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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