IM15L
Anti-uPA (Ab-3) Mouse mAb (16)
lyophilized, clone 16, Calbiochem®
Synonym(s):
Anti-Urkinase Plasminogen Activator
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About This Item
UNSPSC Code:
12352203
biological source
mouse
Quality Level
antibody product type
primary antibodies
clone
16, monoclonal
form
lyophilized
does not contain
preservative
species reactivity
human
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
isotype
IgG1
shipped in
ambient
storage temp.
2-8°C
General description
Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells. Recognizes the B chain of uPA.
Recognizes uPA/PAI complexes in dexamethasone-treated HT-1080 cells and carcinoma tissue. Sold under license of U.S. Patent 5,422,245.
Immunogen
Human
the B chain of human urokinase plasminogen activator
Application
Immunoblotting (2.5 µg/ml, see application references)
Immunoprecipitation (1 µg, see application references)
Immunoprecipitation (1 µg, see application references)
Physical form
Lyophilized from 20 mM NH₄HCO₃, 100 µg BSA.
Preparation Note
We recommend resuspending the lyophilized antibody with sterile phosphate buffered saline (PBS), pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml. Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Following reconstitution, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles.
Analysis Note
Positive Control
Concentrated conditioned medium from dexamethasone-treated HT-1080 cells
Concentrated conditioned medium from dexamethasone-treated HT-1080 cells
Other Notes
Rosenquist, C., et al. 1993. Breast Cancer Res. and Treatment28, 223.
Foekens, J. A., et al. 1992. Cancer Res.52, 6101.
Andreasen, P. A., et al. 1990. Mol. Cell. Endocrinol.68, 1.
Blasi, F. and Verde, P. 1990. in Seminars in Cancer Biology, ed. M. M. Gottesman. Vol. 1, 153.
Duffy, M. J., et al. 1990. Cancer Res.50, 6827.
Jänicke, F., et al. 1990. Fibrinolysis4, 69.
Blasi, F., et al. 1987. J. Cell Biol.104, 801.
Foekens, J. A., et al. 1992. Cancer Res.52, 6101.
Andreasen, P. A., et al. 1990. Mol. Cell. Endocrinol.68, 1.
Blasi, F. and Verde, P. 1990. in Seminars in Cancer Biology, ed. M. M. Gottesman. Vol. 1, 153.
Duffy, M. J., et al. 1990. Cancer Res.50, 6827.
Jänicke, F., et al. 1990. Fibrinolysis4, 69.
Blasi, F., et al. 1987. J. Cell Biol.104, 801.
uPA (Ab-3) will recognize uPA in complex with PAI 1. For optimal results in immunoblotting, incubate HT 1080 cells in serum free media with 1 µM dexamethasone for 2 h and concentrate 10-20X. Antibody should be titrated for optimal results in individual systems.
Legal Information
Sold under license of U.S. Patent 5,422,245.
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
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