Anti-DYNLT3 Antibody, clone 10A3C8A1

Eukaryotic cells rely on actin and microtubule-based protein "motors" to generate intracellular movements. These motor proteins contain specialized domains that hydrolyse ATP to produce force and movement along a cytoskeletal polymer (microtubules in the case of the kinesin family and dyneins) In all cell types, dynein is a large protein complex composed of six distinct subunits and is responsible for most intracellular movement toward the minus ends of microtubules. Cytoplasmic dynein has many important roles in transporting membrane bound organelles, including endosomes, lysosomes, and mitochondria. It is also involved in the positioning of the centrosome, the nucleus, and the Golgi. Cytoplasmic dynein is also responsible for virus transport to the nucleus, retrograde axonal transport, and microtubule and neurofilament transport.
Three families of dynein light chains (DYNLT) have been identified. Two isoforms of the DYNLT family of light chains, DYNLT1 and DYNLT3, have been proposed to link dynein to specific cargoes. Recent publications indicate that DYNLT3 binds to Bub3, a spindle checkpoint protein and Bub3 binds exclusively to DYNLT3 and not to other dynein light chains. DYNLT3 is present on kinetochores at prometaphase, but not later mitotic stages.It has been suggested that a subpopulation of dynein, containing the DYNLT3 light chain, may have a role in the transport of checkpoint proteins from the kinetochore to the spindle pole, (W.-Ho, 2007).

~13 kDa

Full-length recombinant rat DYNLT3 protein

Research Category
Cell Structure

Research Sub Category
Cytoskeletal Signaling

This Anti-DYNLT3 Antibody, clone 10A3C8A1 is validated for use in WB, IH for the detection of DYNLT3.

Other species have not been tested.

Reacts with rat DYNLT3 (dynein light chain Tctex-type 3).

Format: Purified

Protein G Purified

Purified in 0.1M Tris-Glycine, pH 7.4, 150 mM NaCl with 0.05% NaN3.

Maintain at 2-8°C in undiluted aliquots for up to 1 year after date of receipt.

Control
Rat brain lysate

Evaluated on a representative lot by Western blot in rat brain lysate.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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