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Merck

MAB2013

Sigma-Aldrich

Anti-K-Cadherin Antibody, extracellular domain, clone 2B6

clone 2B6, Chemicon®, from mouse

Synonym(s):

Cadherin-6

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702

biological source

mouse

Quality Level

antibody product type

primary antibodies

clone

2B6, monoclonal

species reactivity

human, rat

manufacturer/tradename

Chemicon®

technique(s)

immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable

isotype

IgG1

NCBI accession no.

UniProt accession no.

shipped in

wet ice

Gene Information

human ... CDH6(1004)

General description

Cadherins constitute a family of transmembrane glycoproteins involved in Ca2+-dependent cell-cell interactions. The members of this family are differentially expressed in various tissues. They function in the maintenance of tissue integrity and morphogenesis. The cadherins generally contain five extracellular repeats, a transmembrane domain and a cytoplasmic tail that binds to the catenin family of cytoskeletal anchoring proteins which also function as signal transducers. The extracellular domains are responsible for the specificity of homophilic interactions between cells expressing the same cadherin. Cadherins are divided into type I and type II subgroups. Type I cadherins include epithelial cadherin (E-cadherin, cadherin-1 or uvomorulin), neural cadherin (N-cadherin or cadherin-2), placental cadherin (P-cadherin or cadherin-3) and retinal cadherin (R-cadherin or cadherin-4). Kidney cadherin (K-cadherin or cadherin-6) and osteoblast cadherin (OB-cadherin or cadherin-11) are type II cadherins. Cadherin-6 is expressed in the proximal tubule epithelium of the kidney from which most renal cell carcinomas are derived.

Immunogen

Affinity purified extracellular domain of human K-cadherin
Epitope: extracellular domain

Application

Anti-K-Cadherin Antibody, extracellular domain, clone 2B6 detects level of K-Cadherin & has been published & validated for use in WB, IC, IH.
Research Category
Cell Structure
Research Sub Category
Adhesion (CAMs)
Western blot: 1:25 - 1:250
Immunohistochemistry: 1:25 - 1:50; Frozen tissue sections with avidin-biotinylated horseradish peroxidase complex (ABC) as the detection method.

Optimal working dilutions must be determined by the end user.I/

Biochem/physiol Actions

Reacts with the extracellular domain of K-cadherin.

Physical form

Format: Purified
Purified immuoglobulin. Liquid in PBS containing 0.09% sodium azide.

Preparation Note

1 year at 4°C from date of shipment

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Certificates of Analysis (COA)

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