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Key Documents

MAB2052A4

Sigma-Aldrich

Anti-Disialoganglioside GD2 Antibody, clone 14G2a, Alexa Fluor™488 Conj.

clone 14G2a, from mouse, ALEXA FLUOR™ 488

Synonym(s):

Disialoganglioside GD2

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

conjugate

ALEXA FLUOR™ 488

antibody product type

primary antibodies

clone

14G2a, monoclonal

species reactivity

mouse, rat

technique(s)

immunocytochemistry: suitable
immunohistochemistry: suitable

isotype

IgG2a

shipped in

wet ice

General description

GD2 is expressed on the surfaces of a wide range of tumor cells of neuroectodermal origin, including neuroblastoma, melanomas, and other soft tissue sarcomas. Due to its specific expression on tumor cells, GD2 is an attractive target for immunotherapy. Gangliosides are sialic-acid bearing glycolipids and are likely involved in mediating cell signal transduction events. They are important target antigens for antibody dependent cellular cytotoxicity (ADCC) of human melanoma and neuroblastoma cells. Human melanoma cells produce gangliosides, designated as GD2 and GD3, are deposited in the subtratum-attached material and may play a significant role in the melanoma metastatic phenotype.

Application

Detect Disialoganglioside GD2 using this Anti-Disialoganglioside GD2 Antibody, clone 14G2a, AlexaFluor488 Conj. validated for use in IC, IH.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Disialoganglioside GD2 in N1E-115 Neuroblastoma cells.
Research Category
Neuroscience
Research Sub Category
Developmental Neuroscience

Physical form

Protein A purified
Purified mouse monoclonal IgG2a conjugated to Alexa Fluor™ 488 in PBS with 0.1% sodium azide and 15 mg/mL BSA.

Preparation Note

Maintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt.

Analysis Note

Control
Mouse adult brain tissue
Evaluated by Immunohistochemistry in mouse adult brain tissue.

Immunohistochemistry Analysis: 1:100 dilution of this antibody detected Disialoganglioside GD2 in mouse adult brain tissue.

Other Notes

Unconjugated part (MAB2052) has been shown to work in human.

Legal Information

ALEXA FLUOR is a trademark of Life Technologies

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10-13 - German Storage Class 10 to 13

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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