MAB8173
Anti-EBV EBNA Antibody
Chemicon®, from mouse
Synonym(s):
EBNA-1
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
biological source
mouse
Quality Level
antibody product type
primary antibodies
clone
monoclonal
species reactivity
human
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
immunofluorescence: suitable
western blot: suitable
isotype
IgG1
shipped in
wet ice
Application
Detect EBV EBNA using this Anti-EBV EBNA Antibody validated for use in ELISA, IF & WB.
Western blotting, 1:10-1:50
IFA: 1:10-1:50
ELISA 1:20-1:200
Optimal working dilution must be determined by the end user.
Notes:
Slides/Fixation
cells should be suspended with PBS, pH 7.8 in 200 mM NaCl
air dried onto slides at room temp.
immediately fixed in -20ºC Methanol:Acetone;3:7 (v/v)
air dry slides and store @ 4ºC (<1 month) or -20ºC (6-12 months)
Staining
3 step amplification procedure must be used, i.e.
Mab + goat Anti-mouse IgG + Rabbit Anti-goat/FITC, or
Mab + goat Anti-mouse/biotin + Streptavidin/FITC, or
Mab + guinea pig complement + goat Anti-guinea pig C3/FITC
each incubation should be 45′ at 37ºC
Comments
A 2-step staining procedure is not sensitive enough to detect the EBNA. This is not a function of the monoclonal antibody, but rather a result of the small quantity of EBNA present in the cells.
IFA: 1:10-1:50
ELISA 1:20-1:200
Optimal working dilution must be determined by the end user.
Notes:
Slides/Fixation
cells should be suspended with PBS, pH 7.8 in 200 mM NaCl
air dried onto slides at room temp.
immediately fixed in -20ºC Methanol:Acetone;3:7 (v/v)
air dry slides and store @ 4ºC (<1 month) or -20ºC (6-12 months)
Staining
3 step amplification procedure must be used, i.e.
Mab + goat Anti-mouse IgG + Rabbit Anti-goat/FITC, or
Mab + goat Anti-mouse/biotin + Streptavidin/FITC, or
Mab + guinea pig complement + goat Anti-guinea pig C3/FITC
each incubation should be 45′ at 37ºC
Comments
A 2-step staining procedure is not sensitive enough to detect the EBNA. This is not a function of the monoclonal antibody, but rather a result of the small quantity of EBNA present in the cells.
Biochem/physiol Actions
Specific for the Epstein-Barr Nuclear Antigen (EBNA-1).
Physical form
Format: Purified
Purified immunoglobulin. Liquid in 0.01 M phosphate buffered saline (PBS), pH 7.2, containing 0.1% sodium azide as a preservative.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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