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MABF324

Sigma-Aldrich

Anti-CD8a (mouse) Antibody, Alexa Fluor™647, clone 53-6.7

clone 53-6.7, from rat, ALEXA FLUOR™ 647

Synonym(s):

T-cell surface glycoprotein CD8 alpha chain, T-cell surface glycoprotein Lyt-2, CD8a

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.44

biological source

rat

Quality Level

100

conjugate

ALEXA FLUOR™ 647

antibody product type

primary antibodies

clone

53-6.7, monoclonal

species reactivity

mouse

technique(s)

flow cytometry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable

isotype

IgG2aκ

UniProt accession no.

P01731

shipped in

wet ice

General description

T-cell surface glycoprotein CD8 alpha chain, also known as T-cell surface glycoprotein Lyt-2 or CD8a or CD8a, is a member of the immunoglobulin superfamily acting as a co-receptor with MHC class I-restricted T cell receptors, and through its association with protein tyrosine kinase p56Ick, modulates antigen recognition and T cell activation as well as thymic development. CD8a is expressed on the majority of thymocytes, a subset of mature T lymphocytes, and NK cells (almost exclusively CD8a homodimers).



27 kDa calculated

Application

Anti-CD8a (mouse) Antibody, AlexaFluor647, clone 53-6.7 is a highly specific rat monoclonal antibody, that targets CD8 & has been tested in Flow Cytometry, IHC & IP.

Analysis Note

The antibody was purified by affinity chromatography, and conjugated with AlexaFluor488 under optimal conditions. The solution is free of unconjugated AlexaFluor488 and unconjugated antibody. Alexa Fluor is a registered trademark of Molecular Probes, Inc. Alexa Fluor dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.

Flow Cytometry Analysis: 0.25 μg of this antibody detected CD8a in one million C57BL/6 mouse splenocytes.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

ALEXA FLUOR is a trademark of Life Technologies

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Storage Class Code

10-13 - German Storage Class 10 to 13

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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