MABN828
Anti-LRRTM4 Antibody, clone N205B/22
Synonym(s):
LRRTM4, Leucine-rich repeat transmembrane neuronal protein 4
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About This Item
UNSPSC Code:
12352203
biological source
mouse
clone
N205B/22, monoclonal
species reactivity
human, mouse, rat
isotype
IgG1κ
NCBI accession no.
storage temp.
2-8°C
Gene Information
mouse ... LRRTM4(243499)
General description
Anti-LRRTM4 Antibody, clone N205B/22
Leucine-rich repeat (LRR) containing proteins are involved in protein–protein interactions and mediate cellular events during nervous system development and disease. Human LRRTM4 (NCBI Gene ID 80059) is one of the four LRRTM family of genes that encode leucine-rich repeat transmembrane proteins, orthologous genes exist in murine, rodent, and other vertebrates, but not in invertebrates. LRRTM mRNAs are reported to predominantly express in the nervous system with individual LRRTMs possessing distinct expression patterns (PMID 12676565). The heparan sulfate (HS) proteoglycan glypican is identified as a receptor for LRRTM4, but not LRRTM2. Glypican 4 (GPC4) localized in the presynaptic membranes and LRRTM4 localized in the postsynaptic membranes of excitatory synapses are reported to engage in trans-synaptic interaction, resulting in GPC4 clustering in contacting axons and LRRTM4 clustering in contacting dendrites (PMID 23911103). Clone N205B/22 epitope is 100% conserved among human, murine, and rodent species (UniProt Q86VH4, Q80XG9, B4F7C5, respectively) and is located within the c-terminal region encompassing the last 20% of LTRRTM4 sequence
~70 kDa observed
Immunogen
Conjugated linear peptide corresponding to human LRRTM4
Application
- Western Blotting
- Immunohistochemistry
- Immunocytochemistry
Anti-LRRTM4 Antibody, clone N205B/22 is an antibody against LRRTM4 for use in Western Blotting, Immunohistochemistry and Immunocytochemistry
Western Blotting Analysis: 0.5 µg/mL from a representative lot detected LRRTM4 in 10 µg of rat brain tissue lysate. Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected LRRTM4 in human cerebellum, rat thalamus/Striatum, and rat cerebellum tissue. Immunocytochemistry Analysis: A representative lot detected LRRTM4 in cultured hippocampal neurons
Physical form
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Western Blotting in mouse brain tissue lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected LRRTM4 in 10 µg of mouse brain tissue lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected LRRTM4 in 10 µg of mouse brain tissue lysate.
Other Notes
Concentration : Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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