MABN849

Anti-CaBP1 Antibody, clone 11E7.1

Name: Anti-CaBP1 Antibody, clone 11E7.1
Brand: MM

clone 11E7.1, from mouse

Synonym(s):

Calcium-binding protein 1, CaBP1, Caldendrin

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About This Item

UNSPSC Code:

12352203

eCl@ss:

32160702

NACRES:

NA.41

biological source

mouse

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

11E7.1, monoclonal

species reactivity

mouse, human

technique(s)

immunohistochemistry: suitable (paraffin)
western blot: suitable

isotype

IgG1κ

NCBI accession no.

NP_112482

UniProt accession no.

Q9NZU7

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... CABP1(9478)

General description

Calcium-binding protein 1 (UniProt Q9NZU7; also known as CaBP1, Calbrain, Caldendrin) is encoded by the CABP1 gene (Gene ID 9478) in human. Caldendrin is the founding member of the nCaBP family of neuronal Ca2+-inding proteins, members of which also include CaBP2 through CaBP8. nCaBPs and neuronal calcium sensor (NCS) family members are EF-hand-type calmodulin- (CaM-) like Ca2+-sensor proteins that play important roles in neuronal Ca2+-signaling. In addition to Caldendrin, the CABP1 gene produces three other alternative splicing isoforms, L-CaBP1/Caldendrin-S2, S-CaBP1/Caldendrin-S1, and Calbrain, with varying truncated N-terminal sequence. Caldendrin, L- and S-CaBP1 share four common EF-hands, while the Calbrain isoform contains only the third and fourth EF-hands and lacks the first two. EF-hand 1 constitutively binds Mg2+ and EF-hands 3 and 4 mediate high affinity Ca2+ binding, whereas EF-hand 2 does not bind either Mg2+ or Ca2+. In addition, Caldendrin lacks the N-myristoylation sequence present in L- and S-CaBP1 and many NCS proteins. The unique Caldendrin N-terminal sequence not found in the other spliced isoforms is bipartite in nature, composed of a highly basic proline-rich N-terminal and an acidic C-terminal part. Caldendrin and S-CaBP1 are reported to interact with the L-type voltage-gated calcium channel (VGCC) Cav1.2 via different molecular determinants, and dispaly different effects on Cav1.2 channel activity.

~40 kDa observed. 39.8 kDa (isoform Caldendrin), 25.0 kDa (Isoform L-CaBP1; Caldendrin-S2), 19.4 kDa (Isoform S-CaBP1; Caldendrin-S1), 8.14 kDa (Isoform Calbrain) calculated.

Immunogen

GST-tagged recombinant human CaBP1 internal fragment.

Application

Immunohistochemistry Analysis: An 1:250 dilution from a representative lot detected CaBP1 in human cerebellum and human retina tissue.

This Anti-CaBP1 Antibody, clone 11E7.1 is validated for use in Western Blotting, Immunohistochemistry (Paraffin) for the detection of CaBP1.

Biochem/physiol Actions

Clone 11E7.1 recognizes an internal epitope in spliced isoform Caldendrin (UniProt Q9NZU7-4). The exact location of the epitope has not been mapped. Although not confirmed by direct testing, this monoclonal antibody most likely also reacts with spliced isoform L-CaBP1 (Caldendrin-S2; UniProt Q9NZU7-1), while cross-reactivity toward isoform S-CaBP1 (Caldendrin-S1; UniProt Q9NZU7-2) and isoform Calbrain (UniProt Q9NZU7-3) is unlikely.

Physical form

Format: Purified

Analysis Note

Evaluated by Western Blotting in human brain tissue lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected CaBP1 in 10 µg of human brain tissue lysate.

Other Notes

Concentration: Please refer to lot specific datasheet.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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