NA28
Anti-MLH1 (Ab-1) Mouse mAb (14)
liquid, clone 14, Calbiochem®
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About This Item
UNSPSC Code:
12352203
biological source
mouse
Quality Level
antibody product type
primary antibodies
clone
14, monoclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity
human
should not react with
mouse
manufacturer/tradename
Calbiochem®
storage condition
do not freeze
isotype
IgG1
shipped in
wet ice
storage temp.
−20°C
General description
Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with SP2/0 mouse myeloma cells. Recognizes the ~87 kDa MLH1 protein.
Recognizes the ~83 kDa MLH1 protein in LoVo cells.
This Anti-MLH1 (Ab-1) Mouse mAb (14) is validated for use in Immunoblotting, Paraffin Sections for the detection of MLH1 (Ab-1).
Immunogen
Human
recombinant, human MLH1
Application
Immunoblotting (1 µg/ml)
Paraffin Sections (10 µg/ml, heat pre-treatment required)
Paraffin Sections (10 µg/ml, heat pre-treatment required)
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In 0.05 M sodium phosphate buffer, 0.2% gelatin.
Analysis Note
Negative Control
HCT116 cells
HCT116 cells
Positive Control
LoVo cells or colon tissue
LoVo cells or colon tissue
Other Notes
For paraffin sections the antibody may give a weak immunoreactive staining pattern; high-pressure heat-induced antigen retrieval method is recommended using high/low pH citrate buffer. Antibody should be titrated for optimal results in individual systems.
Kane, M.F., et al. 1997. Cancer Res.57, 808.
Bronner, C.E., et al. 1994. Nature368, 258.
Papadopoulos, N., et al. 1994. Science263, 1625.
Peltomäki, P.T. 1994. Annals of Medicine26, 215.
Fishel, R., et al. 1993. Cell75, 1027.
Leach, F.S., et al. 1993. Cell75, 1215.
Lindbolm, A., et al. 1993. Nature Genetics5, 279.
Bronner, C.E., et al. 1994. Nature368, 258.
Papadopoulos, N., et al. 1994. Science263, 1625.
Peltomäki, P.T. 1994. Annals of Medicine26, 215.
Fishel, R., et al. 1993. Cell75, 1027.
Leach, F.S., et al. 1993. Cell75, 1215.
Lindbolm, A., et al. 1993. Nature Genetics5, 279.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
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Storage Class Code
10-13 - German Storage Class 10 to 13
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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T Fujiwara et al.
The American journal of pathology, 153(4), 1063-1078 (1998-10-20)
A subset of hereditary and sporadic colorectal carcinomas is defined by microsatellite instability (MSI), but the spectra of gene mutations have not been characterized extensively. Thirty-nine hereditary nonpolyposis colorectal cancer syndrome carcinomas (HNPCCa) and 57 sporadic right-sided colonic carcinomas (SRSCCa)
Christophe Papin et al.
Journal of experimental & clinical cancer research : CR, 42(1), 301-301 (2023-11-14)
Inherited defects in the base-excision repair gene MBD4 predispose individuals to adenomatous polyposis and colorectal cancer, which is characterized by an accumulation of C > T transitions resulting from spontaneous deamination of 5'-methylcytosine. Here, we have investigated the potential role of MBD4
Samar Hassen et al.
Journal of experimental & clinical cancer research : CR, 30, 100-100 (2011-10-25)
A broad population-based assay to detect individuals with Lynch Syndrome (LS) before they develop cancer would save lives and healthcare dollars via cancer prevention. LS is caused by a germline mutation in a DNA mismatch repair (MMR) gene, especially protein
Gregoriy A Dokshin et al.
Developmental cell, 52(1), 53-68 (2019-12-17)
GCNA proteins are expressed across eukarya in pluripotent cells and have conserved functions in fertility. GCNA homologs Spartan (DVC-1) and Wss1 resolve DNA-protein crosslinks (DPCs), including Topoisomerase-DNA adducts, during DNA replication. Here, we show that GCNA mutants in mouse and
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