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NA35

Sigma-Aldrich

Anti-p27kip-1 (Ab-2) Mouse mAb (DCS72)

liquid, clone DCS72, Calbiochem®

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About This Item

UNSPSC Code:
12352203

biological source

mouse

Quality Level

100

antibody product type

primary antibodies

clone

DCS72, monoclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

human, mouse

manufacturer/tradename

Calbiochem®

storage condition

do not freeze

isotype

IgG1

shipped in

wet ice

storage temp.

2-8°C

General description

This product has been discontinued.



Recognizes the ~27 kDa p27kip-1 protein in HeLa cells and normal breast tissue.

Purified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with Sp2/0 mouse myeloma cells. Recognizes the ~27 kDa p27kip-1 protein.

Immunogen

recombinant p27Kip-1

Application

Immunoblotting (1 µg/ml, chemiluminescence)

Paraffin Sections (1 µg/ml, heat pre-treatment required; see application references)

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In 50 mM sodium phosphate buffer, 2% gelatin, pH 7.5.

Analysis Note

Negative Control
MOPC21 cells
Positive Control
HeLa cells or normal breast tissue

Other Notes

For staining paraffin sections, tissue should be boiled in 0.01% citric acid in a microwave for 15 min and allowed to cool prior to staining (see application references). Antibody should be titrated for optimal results in individual systems.
Kawamata, N., et al. 1995 Cancer Res.55, 2266.
Pietenpol, J.A., et al. 1995 Canere Res.55, 1206.
Ponce-Casteneda, M.V., et al. 1995 Cancer Res.55, 1211.
Polyak, K., et al. 1994 Genes Dev.8, 9.
Toyoshima, H. and Hunter, T. 1994 Cell78, 67.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

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