Anti-Polypyrimidine Tract Binding Protein (Ab-1) Mouse mAb (SH54)

Polypyrimidine tract binding protein (PTB) is an abundant vertebrate hnRNP protein that functions as a splicing regulator with multiple targets. PTB exists as a 57 kDa protein that is found exclusively in the perinucleolar compartment (PNC) and regulates alternative splicing by binding to a specific pyrimidine tract sequence that is typically found near the 3′ end of introns. The PNC in turn is a structure that is more prevalent in cancer cells than in normal cells. This latter observation suggests the possibility of using expression of PTB as a marker for tumor cells. In solution phase, PTB exists as a homodimer and in some cancer cells exists as two isoforms, the ratio of which appears to correlate with metastatic potential particularly in prostatic carcinoma. PTB is involved in the regulation of many different viral genomes such as that of hepatitis C virus.

Purified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with SP2/0 mouse myeloma cells (see application references). Recognizes the ~57 kDa PTB protein.

Recognizes ~57 kDa PTB protein in Jurkat cells.

This Anti-Polypyrimidine Tract Binding Protein (Ab-1) Mouse mAb (SH54) is validated for use in Immunoblotting, IF, Paraffin Sections for the detection of Polypyrimidine Tract Binding Protein (Ab-1).

HeLa cell nuclear extract

Immunoblotting (1 μg/ml)
Immunofluorescence (1-2 μg/ml, see application references)
Paraffin Sections (2.5 μg/ml, heat pre-treatment required see application references)

In 50 mM sodium phosphate, 0.2% gelatin.

Positive Control
Jurkat cells or breast carcinoma tissue

Antibody should be titrated for optimal results in individual systems.