Key Documents
NE1020
Anti-Myelin CNPase Mouse mAb (SMI-91)
liquid, clone SMI-91, Calbiochem®
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About This Item
UNSPSC Code:
12352203
biological source
mouse
Quality Level
antibody product type
primary antibodies
clone
SMI-91, monoclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity
bovine, porcine, rat, human, sheep, canine, mouse
should not react with
guinea pig, avian
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
avoid repeated freeze/thaw cycles
isotype
IgG1
shipped in
wet ice
storage temp.
−20°C
General description
This product has been discontinued.
Recognizes the ~46 and ~48 kDa subunits of the 94 kDa myelin CNPase dimer in rat brain extracts.
Mouse monoclonal antibody supplied as undiluted ascites. Recognizes the ~46 kDa and ~48 kDa subunits of the 94 kDa myelin CNPase protein.
Immunogen
Human
purified, human myelin CNPase
Application
ELISA (1:200)
Frozen Sections (1:200, see comments)
Immunoblotting (1:200)
Immunocytochemistry (1:200, see comments)
Paraffin Sections (1:200, heat pre-treatment required, see comments)
Physical form
Undiluted ascites.
Preparation Note
Following initial thaw, aliquot and freeze (-20°C).
Analysis Note
Positive Control
Rat brain
Rat brain
Other Notes
Recognizes developing and adult myelin and developing oligodendrocytes and Schwann cells. Distinguishes oligodendrocytes from astrocytes, microglia, neurons, and other cells in brain tissue. In conjunction with Anti-Myelin Basic Protein Mouse mAb (SMI-94) (Cat. No. NE1018) and/or Anti-Myelin Basic Protein Mouse mAb (SMI-99) (Cat. No. NE1019), this antibody is useful for immunocytochemical studies on the progression of normal and pathologic myelination. Tissues can be fixed in a variety of paraformaldehyde- or formaldehyde-containing fixatives. Post-fixation in cold methanol, methanol/hydrogen peroxide, or detergent is necessary for access of the antibody to oligodendrocytes and myelin in frozen tissue sections and cultured cells. For staining paraffin sections it is recommended that de-paraffinized tissues be autoclaved in dH2O for 10 min or boiled in Tris-saline, pH 9.0, in a microwave for 15 min to expose the epitope. Antibody should be titrated for optimal results in individual systems.
Sprinkle, T.J. 1989. Crit. Rev. Neurobiol.4, 235.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
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Storage Class Code
10-13 - German Storage Class 10 to 13
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Chao Weng et al.
Molecular medicine reports, 16(2), 1864-1870 (2017-06-29)
Transcription factor 7 like 2 (TCF7L2, also termed TCF4), is a Wnt effector induced transiently in the oligodendroglial lineage. The current well accepted hypothesis is that TCF7L2 inhibits oligodendrocyte differentiation and remyelination through canonical Wnt/β‑catenin signaling. However, recent studies indicated
Julian Curiel et al.
Human molecular genetics, 26(22), 4506-4518 (2017-10-04)
Hypomyelinating leukodystrophies are heritable disorders defined by lack of development of brain myelin, but the cellular mechanisms of hypomyelination are often poorly understood. Mutations in TUBB4A, encoding the tubulin isoform tubulin beta class IVA (Tubb4a), result in the symptom complex
Di Xie et al.
Journal of neuroinflammation, 17(1), 57-57 (2020-02-18)
Microglia activation is associated with the development of hypoxic-ischemic brain injury (HIBI). Neuroinflammation suppression might be a suitable therapeutic target in hypoxic oligodendrocyte injury. This study aims to determine whether clemastine can improve hypomyelination by suppressing the activated microglia and
Marija Adzic et al.
Journal of neuroscience research, 95(4), 1053-1066 (2016-10-08)
It is widely accepted that adenosine triphosphate (ATP) acts as a universal danger-associated molecular pattern with several known mechanisms for immune cell activation. In the central nervous system, ATP activates microglia and astrocytes and induces a neuroinflammatory response. The aim
Jinsoo Oh et al.
Oncology reports, 41(1), 361-368 (2018-10-27)
Gliomas, the most highly malignant central nervous system tumors, are associated with an extremely poor patient survival rate. Given that gliomas are derived from mutations in glial precursor cells, a considerable number of them strongly react with glial precursor cell‑specific
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