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OP02

Sigma-Aldrich

Anti-v-Fes Rat mAb (F-113)

liquid, clone F-113, Calbiochem®

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About This Item

UNSPSC Code:
12352203

biological source

rat

Quality Level

100

antibody product type

primary antibodies

clone

F-113, monoclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

mouse, rat, human

manufacturer/tradename

Calbiochem®

storage condition

do not freeze

isotype

IgM

shipped in

wet ice

storage temp.

2-8°C

General description

This product has been discontinued.



Recognizes with the v-Fes (feline sarcoma) domain common to the Gardner and Snyder-Thelien FeSv polyprotein translation products and mammalian p93c-fes.

  • Antibody Target Gene Symbol: ABL1
  • Target Synonym: ABL, AI325092, bcr/abl, C-ABL, C-ABL 1B, CABL1, E430008G22Rik, JTK7, MGC117749, p145Abl, p150, v-abl
  • Entrez Gene Name: c-abl oncogene 1, receptor tyrosine kinase
  • Hu Entrez ID: 25

  • Mu Entrez ID: 11350
  • Rat Entrez ID: 311860
    • Purified rat monoclonal antibody generated by immunizing rats with the specified immunogen and fusing splenocytes with Y3 Ag 1.2.3 rat myeloma cells (see application references). Recognizes the v-fes encoded sequences common to the polyprotein translational products of the Snyder-Theilen and Gardner strains of feline sarcoma virus (FesV).

    Immunogen

    v-Fes-transformed cells

    Application

    Immunoprecipitation (1-2 µg/sample)

    Neutralization (see application references)

    Packaging

    Please refer to vial label for lot-specific concentration.

    Physical form

    In 0.05 M sodium phosphate buffer, 0.2% gelatin, pH 7.5.

    Analysis Note

    Negative Control
    FRE cells
    Positive Control
    STFeSVFRECI 10 cells

    Other Notes

    To immunoprecipitate, use goat anti-rat Ig with protein G or rabbit anti-rat Ig in conjunctuction with protein A. This antibody will inhibit protein tyrosine kinase activity (see application references). Antibody should be titrated for optimal results in individual Systems.
    Yu, Gang, et al. 1989. J. Biol. Chem.264, 10276.
    Harper, M.E., et al. 1983. Nature304, 169.
    Dalla-Favera, R., et al. 1982. Proc. Natl. Acad. Sci.79, 4714.
    Groffen, J., et al. 1982. Science216, 1136.
    Veronese, F., et al. 1982. J. Virol.43, 896.
    Barbacid, M., et al. 1980. Proc. Natl. Acad. Sci.77, 5158.
    Sherr, C.J., et al. 1980. J. Virol.34, 200.
    van de Ven, W.J.M., et al. 1980. Virology101, 185.

    Legal Information

    CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

    Disclaimer

    Toxicity: Standard Handling (A)

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