Anti-p73 (Ab-1) Mouse mAb (ER-13)

Purified mouse monoclonal generated by immunizing RBF/DnJ mice with the specified immunogen and fusing splenocytes to NS1 myeloma cells (see application references). Recognizes the ~80 kDa α isoform of p73 protein.

Recognizes the ~80 kDa α isoform of p73 in 293, LOS, and IM09 cells.

The p73 protein shares considerable structural and functional similarity to p53. The p73 gene encodes 4 polypeptides through alternative splicing of exons 11-13 with p73α and p73β expressed in all tissues while expression of p73γ and p73δ may be more restricted. All isoforms show significant sequence identity to the transactivation, DNA binding, and oligomerization domains of p53, and many of the critical amino acid residues in p53 essential for DNA binding are conserved in p73. Not surprisingly, p73 binds to a p53 consensus sequence and transactivates many, but not all, p53-responsive genes. Overproduction of p73 leads to growth arrest and induction of apoptosis in a p53-independent manner. However, p73 does not transactivate all p53-responsive genes and the level of transactivation is often lower than that by p53; thus, the mechanisms of growth arrest and induction of apoptosis by p73 are at least partially distinct from p53. p73 does not appear to be targeted by many of the viral oncoproteins such as E1B 55K, SV40 T antigen, and HPV E6 that bind to and inactivate p53, however both p53 and p73 do bind the Adenovirus E4orf6 protein, suggesting that p73 may be independently inactivated by viral oncoproteins. All p73 isoforms bind one another and p73α and p73β appear to bind p53 as well. Interestingly, mutant p53 binds p73α and inhibits transcriptional activation by p73 indicating that mutational inactivation of p53 may serve to simultaneously inactivate p73. One key difference between p53 and p73 is that, unlike p53, p73 does not appear to be activated in response to DNA damage.

This Anti-p73 (Ab-1) Mouse mAb (ER-13) is validated for use in Gel Shift, Immunoblotting, Immunoprecipitation for the detection of p73 (Ab-1).

Epitope: within amino acids 495-637 of human p73α

recombinant, human p73α protein

Gel Shift (2-4 μg, see application references)
Immunoblotting (2-4 μg/ml)
Immunoprecipitation (2-4 μg)

In 0.05 M sodium phosphate buffer, 0.2% gelatin.

Negative Control
SK-N-AS cells

Positive Control
293, COS, or IM9 cells

Two p73 isoforms are expressed in most cells. p73α migrates as an ~80 kDa protein and p73β migrates as a 68 kDa protein. Will detect p73α in immunoblots and will immunoprecipitate p73α and a p73α/p73β complex. This antibody w