Anti-c-Myc (Ab-1) Mouse mAb (9E10) Fluorescein Conjugate

Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with SP2/0 mouse myeloma cells. Conjugated to FITC. Recognizes the ~64-67 kDa (apparent MW) c-Myc protein.

Recognizes the ~60-67 kDa c-Myc protein in HL60 cells and lung carcinoma tissue.

This Anti-c-Myc (Ab-1) Mouse mAb (9E10) Fluorescein Conjugate is validated for use in FC, Frozen Sections, Immunoblotting, IF, IP, Paraffin Sections for the detection of c-Myc (Ab-1).

Epitope: within amino acids 410-419

Human

a synthetic peptide (AEEQKLISEEDLLRKRREQLKHKLEQLRNSCA) corresponding to amino acids 408-439 of human c-Myc

Flow Cytometry (20 µg/ml)

Frozen Sections (use Cat. No. OP10, OP10L, or OP10T)

Immunoblotting (use Cat. No. OP10, OP10L, or OP10T)

Immunofluorescence (1-5 µg/ml)

Immunoprecipitation (use Cat. No. OP10, OP10L, or OP10T)

Paraffin Sections (use Cat. No. OP10, OP10L, or OP10T)

Please refer to vial label for lot-specific concentration.

In 50 mM sodium phosphate buffer, 0.2% gelatin.

Negative Control
HT1080 cells

Positive Control
HL-60 cells or lung carcinoma

LeGouy, E., et al. 1987. In Nuclear Oncogenes, Cold Spring Harbor Laboratory, 144.
Cole, M.D. 1986. Ann. Rev. Gen.20, 361.
Nisen, P.D., et al. 1986. Cancer Res.46, 6217.
Nau, M.M., et al. 1985. Nature318, 69.
Persson, H., et al. 1984. Science225, 687.
Alitalo, K., et al. 1983. Proc. Natl. Acad. Sci. USA80, 1707.

The c-Myc protein is extremely labile. c-Myc degradation can be as a result of freeze/thaw cycles, thus, we recommend using fresh lysates with a cocktail of protease inhibitors. For immunoblotting, paraffin sections (colorimetric) Cat. No. OP10, OP10L, and OP10F are available. c-Myc is often destroyed during the processing of paraffin sections and can therefore be difficult to detect in this application. This antibody recognizes the c-Myc protein and its cleavage products. The sequence of c-Myc predicts a ~45 kDa protein, but c-Myc migrates under reducing conditions as a ~64-67 kDa band. This antibody will not detect v-Myc but may react weakly to rodent c-Myc when used at high concentrations (10 µg/ml). An extra ~34-40 kDa band may be detected in a immunoblot. May also be used in immunofluorescence as well as the detection and purification of recombinant proteins tagged with the Myc epitope sequence EQKLISEEDL (see application references). The immunogen, c-Myc (Peptide-1) is also available for competition studies (Cat. No. PP06). Antibody should be titrated for optimal results in individual systems.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Toxicity: Standard Handling (A)