Anti-CD63 Mouse mAb (NKI/C-3)

Anti-CD63, mouse monoclonal, clone NKI/C-3, recognizes the heterogeneous 25-110 kDa CD63 in melanomas, clear cell sarcomas, and normal melanocytes. It is validated for WB, & IHC on paraffin sections.

CD63 is a glycosyl phosphatidyl inositol (GPI)-anchored, granulocyte-specific activation antigen whose expression is up-regulated in activated neutrophils. It exists as a heterogeneous 25-110 kDa glycoprotein that is located mainly in the innerside of membranes of cytoplasmic vesicles in melanoma cells. The unglycosylated 25 kDa precursor can be detected in tissue sections by immunohistochemistry. CD63 can be detected in melanomas, clear cell sarcomas (melanoma of soft tissue), nevocellular nevi, and normal melanocytes. It has also been detected in some mucus producing tumors, carcinoids, carcinomas of the thyroid, mast cells, histiocytes in tumor regions, and in secretory cells such as salivary glands, bronchial glands, sweat glands, pancreas, and prostate.

Protein G purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with mouse myeloma P3X62Ag8 cells. Recognizes the heterogeneous ~25-110 kDa CD63 glycoprotein.

Recognizes the heterogeneous 25-110 kDa CD63 glycoprotein in melanomas, clear cell sarcomas, nevocellular nevi, and normal melanocytes. Also reacts with some mucous-producing tumors, carcinoids, carcinomas of the thyroid, mast cells, histiocytes in tumor regions, and cells with secretory function.

plasma membrane fraction of human MeWo cells

Immunoblotting (see application references)
Paraffin Sections (0.2-0.4 μg/ml, heat pre-treatment required; see application references)

In 10 mM PBS, 0.2% BSA, pH 7.4.

Positive Control
Melanoma tissue

Exhibits cytoplasmic staining when used in immunohistochemistry. Staining of formalin-fixed, paraffin-embedded tissue sections requires boiling in 10 mM citrate buffer, pH 6.0, for 10-20 min, followed by cooling at room temperature for 20 min. Antibody sh