Anti-c-N-ras (Ab-1) Mouse mAb (F155-227)

Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with P3X63 Ag8.653 myeloma cells. Recognizes the ~21 kDa c-N-ras protein.

Recognizes the ~21 kDa c-N-Ras protein in neuronal cells. Does not react with c-H-Ras or c-K-Ras under conditions tested.

The human ras gene family consists of three identified members, H, K and N-ras, encoding proteins of 188-189 amino acids and 21,000 (p21) daltons. Human H- and K-ras are the homologues of v-H- and v-K-ras sequences originally isolated from Harvey and Kirsten strains of rat sarcoma viruses. Normal human cellular ras genes can be activated to oncogenes by mutations occurring in codons 12, 13, and 61; such mutated, activated and transforming ras genes have been identified and isolated from human tumors and cultured tumor cells. Although the expression patterns of ras proto-oncogene proteins in normal human tissues are known, similar information for activated ras oncogene encoded p21s and their relevance to human disease diagnosis and prognosis is still emerging.

recombinant p21 Ras

Frozen Sections (10 μg/ml)
Immunoblotting (1-10 μg/ml)
Immunoprecipitation (see comments)
Paraffin Sections (10 μg/ml, saponin or pepsin pre-treatment required)

In 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.

Positive Control
Neuronal cells

The level of expression of p21ras is variable in different tissues. For this reason a concentration step such as immunoprecepitation prior to immunoblot analysis is recommended to obtain optimal results. c-N-ras (Ab-1) reacts with c-N-ras and v-N-ras but not with c-H-ras or c-K-ras, under the conditions tested. For immunoprecipitation, use 5 μg antibody per reaction with 45 μl of Protein G plus-agarose (Cat. No. IP04). Antibody should be titrated for optimal results in individual systems.