OP53
Anti-c-Fos (Ab-3) Mouse mAb (CS93.1.18)
liquid, clone CS93.1.18, Calbiochem®
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About This Item
UNSPSC Code:
12352203
biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
CS93.1.18, monoclonal
form
liquid
does not contain
preservative
species reactivity
mouse, human
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
isotype
IgG1
shipped in
wet ice
storage temp.
−20°C
Gene Information
human ... FOS(2353)
General description
Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with SP2/0-AG14 plasmacytoma cells. Recognizes the ~50-62 kDa Fos protein.
Recognizes the ~50-62 kDa c-Fos protein in serum-stimulated NIH3T3 cells and A431 cells. Also recognizes v-fos.
This Anti-c-Fos (Ab-3) Mouse mAb (CS93.1.18) is validated for use in Immunoblotting, Paraffin Sections for the detection of c-Fos (Ab-3).
Immunogen
Human
a synthetic peptide (SGFNADYEASSSRC) (Cat. No. PP10) corresponding to amino acids 4-17 of human c-Fos
Application
Immunoblotting (1-5 µg/ml)
Paraffin Sections (1-10 µg/ml; pepsin pre-treatment required)
Paraffin Sections (1-10 µg/ml; pepsin pre-treatment required)
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In 0.05 M sodium phosphate buffer, 50% glycerol.
Preparation Note
Following initial thaw, aliquot and freeze (-20°C).
Analysis Note
Negative Control
Growth-arrested cells
Growth-arrested cells
Positive Control
Serum-stimulated NIH 3T3 cells or A431 cells
Serum-stimulated NIH 3T3 cells or A431 cells
Other Notes
Rouscher III, F.J., et al. 1988. Science240, 1010.
Sassone-Corsi, P., et al. 1988. Cell54, 553.
Verma, I.M., and Graham, W.R.,1987. Adv. Cancer Res.49, 29.
Verma, I.M., and Sassone-Corsi, P., 1987. Cell52, 513).
Muller, R., 1986. Biochim. Biophys. Acta823, 207.
Verma, I.M., 1986. Trends Genet.2, 93.
Greenberg, M., and Ziff, E., 1984. Nature, London311, 433.
Sassone-Corsi, P., et al. 1988. Cell54, 553.
Verma, I.M., and Graham, W.R.,1987. Adv. Cancer Res.49, 29.
Verma, I.M., and Sassone-Corsi, P., 1987. Cell52, 513).
Muller, R., 1986. Biochim. Biophys. Acta823, 207.
Verma, I.M., 1986. Trends Genet.2, 93.
Greenberg, M., and Ziff, E., 1984. Nature, London311, 433.
This antibody will react with both cellular and viral forms of Fos. The immunogen c-Fos (Peptide-2), Cat. No. PP10, is also available for competition studies. Antibody should be titrated for optimal results in individual systems.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
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