Anti-PKC (Ab-2) Mouse mAb (MC5)

Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with X-63 mouse myeloma cells. Recognizes ~79-80 kDa α, β1, and β2 isoforms of protein kinase C.

Recognizes the ~79-80 kDa protein kinase C α/β1/β2 isoforms in NIH3T3 cells and brain tissue.

The protein kinase C (PKC) family of protein consists of 12 known isozyme members which are classified as Ca⁺⁺/phosphatidylserine-dependent, serine/threonine kinases. In addition to their involvement in signal transduction they are also involved with cell proliferation, differentiation and function. PKCγ is found primarily in the brain and spinal cord. Expression in other tissues including epidermal, adrenal, haematopoietic, and some tumors is low or variable.

This Anti-PKC (Ab-2) Mouse mAb (MC5) is validated for use in Blocking Studies, Immunoblotting, Immunoprecipitation, Paraffin Sections for the detection of PKC (Ab-2).

Epitope: within the hinge region of PKC

purified bovine brain PKC

Blocking Studies (5 μg/ml, see comments)
Immunoblotting (1 μg/ml)
Immunoprecipitation (see application references)
Paraffin Sections (5 μg/ml, no pre-treatment required)

In 50 mM sodium phosphate buffer, 0.2% gelatin.

Positive Control
NIH3T3 cells or brain tissue

May be used to block the down regulation of PKC activity. Antibody should be titrated for optimal results in individual systems.