Key Documents
OP76
Anti-p21WAF1 (Ab-4) Mouse mAb (65)
liquid, clone 65, Calbiochem®
Synonym(s):
Anti-CIP1, Anti-SD11, Anti-p21, Anti-WAF
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About This Item
UNSPSC Code:
12352203
biological source
mouse
Quality Level
antibody product type
primary antibodies
clone
65, monoclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity
rat, mouse
manufacturer/tradename
Calbiochem®
storage condition
do not freeze
isotype
IgG1
shipped in
wet ice
storage temp.
2-8°C
General description
This product has been discontinued.
Recognizes the ~21 kDa p21WAF1 protein in rat and mouse embryo fibroblast cell lines treated with actinomycin D.
Purified mouse monoclonal antibody. Recognizes ~21 kDa p21WAF1 protein.
Immunogen
Epitope: within amino acids 90-109 of mouse p21WAF1
recombinant, mouse p21WAF1
Application
Immunoblotting (3 µg/ml, chemiluminescence)
Immunoprecipitation (2 µg/sample)
Immunoprecipitation (2 µg/sample)
Packaging
Please refer to vial label for lot-specific concentration.
Analysis Note
Positive Control
Rat or mouse embryo fibroblast cell lines treated with Actinomycin D (Cat. No. 114666)
Rat or mouse embryo fibroblast cell lines treated with Actinomycin D (Cat. No. 114666)
Other Notes
Agarwal, M.L., et al. 1995. Proc. Natl. Acad. Sci. USA92, 8493.
Chen, Y.Q., et al. 1995. Int. J. Oncology7, 889.
Deng, C., et al. 1995. Cell82, 675.
El-Deiry, W.S., et al. 1995. Cancer Res.55, 2910.
Waldman, T., et al. 1995. Cancer Res.55, 5187.
Elbendary, A., et al. 1994. Cell Growth Diff.5, 1301.
El-Deiry, W.S., et al. 1994. Cancer Res.54, 1169.
Li, R., et al. 1994. Nature371, 534.
Michieli, P., et al. 1994. Cancer Res.54, 3391.
Noda, A., et al. 1994. Exp. Cell Res.211, 90.
El-Deiry, W.S., et al. 1993. Cell75, 817.
Gu, Y., et al. 1993. Nature366, 707.
Harper, J.W., et al. 1993. Cell75, 805.
Xiong, Y., et al. 1993. Nature366, 701.
Xiong, Y., et al. 1993. Genes Devel.7,1572.
Xiong, Y., et al. 1992. Cell71, 505.
Chen, Y.Q., et al. 1995. Int. J. Oncology7, 889.
Deng, C., et al. 1995. Cell82, 675.
El-Deiry, W.S., et al. 1995. Cancer Res.55, 2910.
Waldman, T., et al. 1995. Cancer Res.55, 5187.
Elbendary, A., et al. 1994. Cell Growth Diff.5, 1301.
El-Deiry, W.S., et al. 1994. Cancer Res.54, 1169.
Li, R., et al. 1994. Nature371, 534.
Michieli, P., et al. 1994. Cancer Res.54, 3391.
Noda, A., et al. 1994. Exp. Cell Res.211, 90.
El-Deiry, W.S., et al. 1993. Cell75, 817.
Gu, Y., et al. 1993. Nature366, 707.
Harper, J.W., et al. 1993. Cell75, 805.
Xiong, Y., et al. 1993. Nature366, 701.
Xiong, Y., et al. 1993. Genes Devel.7,1572.
Xiong, Y., et al. 1992. Cell71, 505.
Maximal p21 WAF1 expression requires wild type p53 activity. Treatment of mouse or rat embryo fibroblasts with DNA damaging agents such as actinomycin D induces wild type p53 expression which in turn activates WAF1 expression. Serum stimulation of quiescent cells will give low level WAF1 expression independent of p53 expression. This antibody can co-precipitate proteins associated with WAF1. Antibody should be titrated for optimal results in individual systems.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
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Storage Class Code
10-13 - German Storage Class 10 to 13
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Thong C Ma et al.
Neurobiology of disease, 49, 13-21 (2012-09-05)
Preventing neuronal death is a priority for treating neurological diseases. However, therapies that inhibit pathological neuron loss could promote tumorigenesis by preventing the physiological death of cancerous cells. To avert this, we targeted the transcriptional upregulation of p21(waf1/cip1) (p21), an
A Ouhtit et al.
The American journal of pathology, 156(1), 201-207 (2000-01-07)
We examined the effects of ultraviolet (UV) radiation on the time course for induction of sunburn (apoptotic) cells and expression of proteins known to be associated with growth arrest and apoptosis in SKH-hr1 mouse skin. Mice were irradiated with a
Ji-Hoon Park et al.
Cancer research, 78(18), 5375-5383 (2018-07-26)
Inheritance of germline mutations in the tumor suppressor gene TP53 causes Li-Fraumeni syndrome (LFS), a cancer predisposition disorder. The arginine to histidine substitution at amino acid position 337 of p53 (R337H) is a founder mutation highly prevalent in southern and
Jie Zhuang et al.
The Journal of biological chemistry, 291(48), 24819-24827 (2016-10-01)
Although exercise is linked with improved health, the specific molecular mechanisms underlying its various benefits require further clarification. Here we report that exercise increases the nuclear localization and activity of p53 by acutely down-regulating coiled-coil-helix-coiled-coil-helix domain 4 (CHCHD4), a carrier
Mitochondrial disulfide relay mediates translocation of p53 and partitions its subcellular activity.
Jie Zhuang et al.
Proceedings of the National Academy of Sciences of the United States of America, 110(43), 17356-17361 (2013-10-09)
p53, a critical tumor suppressor, regulates mitochondrial respiration, but how a nuclear protein can orchestrate the function of an organelle encoded by two separate genomes, both of which require p53 for their integrity, remains unclear. Here we report that the
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