Skip to Content
Merck

Key Documents

OP76

Sigma-Aldrich

Anti-p21WAF1 (Ab-4) Mouse mAb (65)

liquid, clone 65, Calbiochem®

Synonym(s):

Anti-CIP1, Anti-SD11, Anti-p21, Anti-WAF

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

UNSPSC Code:
12352203

biological source

mouse

Quality Level

100

antibody product type

primary antibodies

clone

65, monoclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

rat, mouse

manufacturer/tradename

Calbiochem®

storage condition

do not freeze

isotype

IgG1

shipped in

wet ice

storage temp.

2-8°C

General description

This product has been discontinued.



Recognizes the ~21 kDa p21WAF1 protein in rat and mouse embryo fibroblast cell lines treated with actinomycin D.

Purified mouse monoclonal antibody. Recognizes ~21 kDa p21WAF1 protein.

Immunogen

Epitope: within amino acids 90-109 of mouse p21WAF1
recombinant, mouse p21WAF1

Application

Immunoblotting (3 µg/ml, chemiluminescence)

Immunoprecipitation (2 µg/sample)

Packaging

Please refer to vial label for lot-specific concentration.

Analysis Note

Positive Control
Rat or mouse embryo fibroblast cell lines treated with Actinomycin D (Cat. No. 114666)

Other Notes

Agarwal, M.L., et al. 1995. Proc. Natl. Acad. Sci. USA92, 8493.
Chen, Y.Q., et al. 1995. Int. J. Oncology7, 889.
Deng, C., et al. 1995. Cell82, 675.
El-Deiry, W.S., et al. 1995. Cancer Res.55, 2910.
Waldman, T., et al. 1995. Cancer Res.55, 5187.
Elbendary, A., et al. 1994. Cell Growth Diff.5, 1301.
El-Deiry, W.S., et al. 1994. Cancer Res.54, 1169.
Li, R., et al. 1994. Nature371, 534.
Michieli, P., et al. 1994. Cancer Res.54, 3391.
Noda, A., et al. 1994. Exp. Cell Res.211, 90.
El-Deiry, W.S., et al. 1993. Cell75, 817.
Gu, Y., et al. 1993. Nature366, 707.
Harper, J.W., et al. 1993. Cell75, 805.
Xiong, Y., et al. 1993. Nature366, 701.
Xiong, Y., et al. 1993. Genes Devel.7,1572.
Xiong, Y., et al. 1992. Cell71, 505.
Maximal p21 WAF1 expression requires wild type p53 activity. Treatment of mouse or rat embryo fibroblasts with DNA damaging agents such as actinomycin D induces wild type p53 expression which in turn activates WAF1 expression. Serum stimulation of quiescent cells will give low level WAF1 expression independent of p53 expression. This antibody can co-precipitate proteins associated with WAF1. Antibody should be titrated for optimal results in individual systems.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Not finding the right product?  

Try our Product Selector Tool.

Storage Class Code

10-13 - German Storage Class 10 to 13

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service