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Key Documents

OP79

Sigma-Aldrich

Anti-p21WAF1 (Ab-6) Mouse mAb (22)

liquid, clone 22, Calbiochem®

Synonym(s):

Anti-CIP1, Anti-SD11, Anti-p21, Anti-WAF

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About This Item

UNSPSC Code:
12352203

biological source

mouse

antibody product type

primary antibodies

clone

22, monoclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

mouse, human, rat

manufacturer/tradename

Calbiochem®

storage condition

do not freeze

isotype

IgG1

shipped in

wet ice

storage temp.

2-8°C

General description

This product has been discontinued.



Recognizes the ~21 kDa p21WAF1 protein in rat and mouse embryo fibroblast cell lines treated with actinomycin D.

Purified mouse monoclonal antibody, generated by immunizing BALB/c x C57B1/6 F1 mice with the specified immunogen and fusing splenocytes with myeloma cells. Recognizes the ~21 kDa p21WAF1 protein.

Immunogen

Epitope: within amino acids 20-40 of human p21WAF1
recombinant, mouse p21WAF1

Application

Frozen Sections (not recommended)

Immunoblotting (3 µg/ml)

Immunoprecipitation (1 µg/ml)

Paraffin Sections (not recommended)

Packaging

Please refer to vial label for lot-specific concentration.

Analysis Note

Positive Control
Induced rat or mouse embryo fibroblasts

Other Notes

Agarwal, M.L., et al. 1995. Proc. Natl. Acad. Sci. USA92, 8493.
Chen, Y.Q., et al. 1995. Int. J. Oncology7, 889.
Deng, C., et al. 1995. Cell82, 675.
El-Deiry, W.S., et al. 1995. Cancer Res.55, 2910.
Waldman, T., et al. 1995. Cancer Res.55, 5187.
Elbendary, A., et al. 1994. Cell Growth Diff.5, 1301.
El-Deiry, W.S., et al. 1994. Cancer Res.54, 1169.
Li, R., et al. 1994. Nature371, 534.
Michieli, P., et al. 1994. Cancer Res.54, 3391.
Noda, A., et al. 1994. Exp. Cell Res.211, 90.
El-Deiry, W.S., et al. 1993. Cell75, 817.
Gu, Y., et al. 1993. Nature366, 707.
Harper, J.W., et al. 1993. Cell75, 805.
Xiong, Y., et al. 1993. Nature366, 701.
Xiong, Y., et al. 1993. Genes Devel.7,1572.
Xiong, Y., et al. 1992. Cell71, 505.
Maximal p21WAF1 expression requires wild type p53 activity. Treatment of mouse or rat embryo fibroblasts with DNA damaging agents such as actinomycin D induces wild type p53 expression which in turn activates WAF1 expression. Serum stimulation of quiescent cells will give low level WAF1 expression independent of p53 expression. For immunoblotting applications, antigen/antibody complexes are best visualized using chemiluminescence detection methods. Antibody should be titrated for optimal results in individual systems.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

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Storage Class Code

10-13 - German Storage Class 10 to 13

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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