Key Documents
PC08
Anti-EGF (Ab-3) Rabbit pAb
Synonym(s):
Anti-Epidermal Growth Factor
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About This Item
antibody form
purified antibody
Quality Level
clone
polyclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity
human
isotype
IgG
General description
Epidermal growth factor (EGF) is an acid- and heat-stable, 53 amino acid protein of 6045 MW originally found in rodents and humans. EGF has been shown to be a potent mitogen for a variety of cell types both in vivo and in vitro. It binds to a 170,000 dalton receptor on the surface of cells and mediates intrinsic phosphorylation of the receptor on tyrosine residues. EGF has been detected in nearly all body fluids such as urine (urogastrone) saliva, milk and platelet-rich plasma. EGF, transforming growth factor-α and vaccinia virus growth factor all bind to and activate the EGF receptor and exhibit 30-40% amino acid homology.
Purified rabbit polyclonal antibody. Recognizes the ~6 kDa EGF protein.
Recognizes the EGF protein in salivary gland tissue. Shows no reactivity with human TGFα.
Immunogen
recombinant, human EGF
Application
Immunoblotting (1-5 μg/ml)
Paraffin Sections (0.5-2 μg/ml, saponin pre-treatment required)
Paraffin Sections (0.5-2 μg/ml, saponin pre-treatment required)
Physical form
In 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.4.
Analysis Note
Positive Control
Purified EGF or salivary gland
Purified EGF or salivary gland
Other Notes
Shows no cross-reactivity with human transforming growth factor-α. Antibody should be titrated for optimal results in individual systems.
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Radioimmunoassay of human epidermal growth factor (urogastrone).
R H Starkey et al.
The Journal of clinical endocrinology and metabolism, 45(6), 1144-1153 (1977-12-01)
Y Oka et al.
The Journal of clinical investigation, 72(1), 249-259 (1983-07-01)
Human epidermal growth factor (hEGF) has previously been isolated from urine and probably is identical to human beta-urogastrone (hUG). Immunoreactive hEGF/UG has been found in the plasma of normal subjects. In this study, using immunoaffinity chromatography to extract hEGF/UG from
Receptors for epidermal growth factor and other polypeptide mitogens.
G Carpenter
Annual review of biochemistry, 56, 881-914 (1987-01-01)
G Carpenter et al.
Experimental cell research, 164(1), 1-10 (1986-05-01)
An interesting aspect of the developments forthcoming from the study of cell growth control by growth factors is the structural and functional homologies that have been found to exist between growth factors or their receptors and other molecules. The epidermal
Transforming growth factor-alpha: structure and biological activities.
R Derynck
Journal of cellular biochemistry, 32(4), 293-304 (1986-01-01)
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