Anti-Pan-Ras Asp¹² (Ab-1) Rabbit pAb

Purified rabbit polyclonal antibody. Recognizes the ~21 kDa H-, K-, and N-Ras proteins.

Recognizes the ~21 kDa H-, K-, and N-Ras proteins containing aspartic acid at position 12.

a synthetic peptide (KLVVVGADGVGK) corresponding to amino acids 5-16 of Ras (H, K, or N) with Asp at position 12

Immunoblotting (1-5 µg/ml)

Lyophilized from 20 mM ammonium bicarbonate, 100 µg BSA.

Reconstitute the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4; product will be more stable if 0.1% sodium azide is included (do not add azide if antibody is to be used with viable cells). Lyophilized antibody should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Following reconstitution, aliquot and freeze (-20°C) for long-term storage or refrigerate (4°C) for short-term storage with 0.1% sodium azide. Avoid freeze/thaw cycles of solutions.

Negative Control
SW480 cells

Positive Control
HS578T cells or purified recombinant K-Ras Asp¹² protein

Bos, J.L., et al. 1988. Mutation Res.195, 255.
Furth, M.E., et al. 1987. Oncogene1, 47.
Shimizu, K., et al. 1983. Proc. Natl. Acad. Sci. USA80, 2112.
Taparowsky, E., et al. 1983. Cell34, 581.
Ellis, R.W., et al. 1981. Nature292, 506.
Shih, T.Y., et al. 1990 Nature287, 686.

Does not react with ras^Gly-12 or ras^Val-12 p21′s. Antibody should be titrated for optimal results in individual systems.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Toxicity: Standard Handling (A)