PC21
Anti-PDGF (Ab-1) (101-116) Rabbit pAb
liquid, Calbiochem®
Synonym(s):
Anti-Platelet Derived Growth Factor
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About This Item
UNSPSC Code:
12352203
biological source
rabbit
Quality Level
antibody product type
primary antibodies
clone
polyclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity
human, rat
manufacturer/tradename
Calbiochem®
storage condition
do not freeze
isotype
IgG
shipped in
wet ice
storage temp.
2-8°C
General description
Purified rabbit polyclonal antibody. Recognizes the B form of human PDGF.
Recognizes the B subunit of PDGF in mesenchymal cells and fetal epithelial tissue.
This Anti-PDGF (Ab-1) (101-116) Rabbit pAb is validated for use in Immunoblotting, Immunofluorescence, Paraffin Sections, ELISA for the detection of PDGF (Ab-1) (101-116).
Immunogen
Human
a synthetic peptide (TEVFEISRRLIDRTNA) corresponding amino acids 101-116 of human PDGF B chain
Application
Immunoblotting (1-5 µg/ml)
Immunofluorescence (1-5 µg/ml)
Paraffin Sections (1-10 µg/ml, heat pre-treatment required)
ELISA (see comments)
Immunofluorescence (1-5 µg/ml)
Paraffin Sections (1-10 µg/ml, heat pre-treatment required)
ELISA (see comments)
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In 0.05 M sodium phosphate buffer, 0.2% gelatin.
Analysis Note
Negative Control
PC12 cells
PC12 cells
Positive Control
Mesenchymal cells or fetal epithelial tissue
Mesenchymal cells or fetal epithelial tissue
Other Notes
LaRochelle, W.J., et al. 1989 Mol. Cell. Biol.9, 3538.
Hannink, M., et al. 1986 Mol. Cell. Biol.6, 1304.
Ross, R., et al. 1986 Cell46, 155.
Devare, S.G., et al. 1983 Proc. Natl. Acad. Sci. USA80, 731.
Waterfield, M.D., et al. 1983. Nature (London)304, 35.
Johnsson, A., et al. 1982 Biochem. Biophys. Res. Commun.104, 66.
Hannink, M., et al. 1986 Mol. Cell. Biol.6, 1304.
Ross, R., et al. 1986 Cell46, 155.
Devare, S.G., et al. 1983 Proc. Natl. Acad. Sci. USA80, 731.
Waterfield, M.D., et al. 1983. Nature (London)304, 35.
Johnsson, A., et al. 1982 Biochem. Biophys. Res. Commun.104, 66.
This antibody specific for the B form of human PDGF that will react by immunofluorescence with human platelets and has been reported to react in ELISA with the PDGF(AB) and PDGF(BB) protein. Antibody should be titrated for optimal results in individual sample types.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
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Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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