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Merck

PC246

Sigma-Aldrich

Anti-GluR1 Rabbit pAb

liquid, Calbiochem®

Synonym(s):

Anti-Glutamate Receptor I

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About This Item

UNSPSC Code:
12352203

biological source

rabbit

Quality Level

antibody product type

primary antibodies

clone

polyclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

rat

manufacturer/tradename

Calbiochem®

storage condition

do not freeze

isotype

IgG

shipped in

wet ice

storage temp.

2-8°C

General description

Anti-GluR1, rabbit polyclonal, recognizes the ~105-107 kDa GluR1 in D425 Med cells and hippocampal neurons. Also cross-reacts with a 50 kDa protein in rat brain. It is validated for WB and IF.
Purified rabbit polyclonal antibody. Recognizes the ~105-107 kDa GluR1 protein.
Recognizes the ~105-107 kDa GluR1 protein in D425 Med cells and hippocampal neurons. Also cross-reacts with a 50 kDa protein in rat brain extracts.

Immunogen

a synthetic peptide (RTSDSRDHTRVDWKR) corresponding to amino acids 271-285 of rat GluR1 (numbered from the signal peptide)

Application

Immunoblotting 1-5 µg/ml)

Immunofluorescence (10-20 µg/ml, see application references)

Packaging

Please refer to vial label for lot-specific concentration.

Analysis Note

Positive Control
D425 Med cells, hippocampal neurons

Other Notes

Also recognizes a smaller band at ~50 kDa using whole brain extracts. Immunofluorescence of dissociated cultured rat hippocampal neurons gave a punctate distribution, consistent with previous reports of GluR1 distribution. Antibody should be titrated for optimal results in individual systems.
Zuo, J. et al. 1997. Nature388, 769.
McHugh, T.J., et al. 1996. Cell87, 1339.
Tsien, J.Z., et al. 1996. Cell87, 1327.
Greenamyre, J.T. and Porter, R.H.P. 1994. Neurology44, S7.
Hollmann, M. and Heinemann, S. 1994. Ann. Rev. Neurosci.17, 31.
Bliss, T.V.P. and Collingridge, G.L. 1993. Nature361, 31.
Lomeli, H., et al. 1993. FEBS Lett.315, 318.
Molnar, E. et al. 1993. Neuroscience53, 307.
Advokat, C. and Pellegrin, A.I. 1992. Neurosci. Biobehav. Rev.16, 13.
Beal, M.F. 1992. FASEB J.6, 3338.
Rothman, S.M. 1992. Ann. NY Acad. Sci.648, 132.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

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Storage Class Code

10-13 - German Storage Class 10 to 13


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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