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PC315

Sigma-Aldrich

Anti-VEGF (Ab-4) Rabbit pAb

liquid, Calbiochem®

Synonym(s):

Anti-Vascular Endothelial Growth Factor, Anti-Vascular Permeability Factor, Anti-VPF

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About This Item

UNSPSC Code:
12352203

biological source

rabbit

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

polyclonal

form

liquid

contains

15 mM sodium azide as preservative

species reactivity

human, mouse, rat

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

isotype

IgG

shipped in

wet ice

storage temp.

−20°C

General description

Protein A purified rabbit polyclonal antibody. Recognizes the ~34-50 kDa isoforms of VEGF.
Recognizes the ~34-50 kDa isoforms of VEGF. Cat. No. PC315 is recommended for immunohistochemical staining of rodent tissues. Recognizes VEGF in human tissues by immunoblotting but not by immunohistochemistry.

Immunogen

Human
recombinant, human VEGF₁₆₅

Application


Paraffin Sections (1:200-1:400, pressure cooker pre-treatment required, see comments)

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In 10 mM PBS, 0.2% BSA, pH 7.6.

Preparation Note

Following intitial thaw, aliquot and freeze (-20°C).

Analysis Note

Positive Control
Recombinant VEGF or blood vessels in skin or intestine (rodent only)

Other Notes

Soker, S., et al. 1998. Cell92, 735.
Kroll, J. and Waltenberger, J. 1997. J. Biol. Chem.272, 32521.
Klagsbrun, M. and Soker, S. 1993. Current Biology3, 699.
Senger, D., et al. 1993. Cancer and Metastasis Rev.12, 303.
Brown, L., et al. 1992. Kidney International42, 1457.
Dvorak, H., et al. 1991. J. Exp. Med.174, 1275.
Tested on formalin-fixed, paraffin embedded tissue. Staining of formalin-fixed tissues requires boiling tissue sections in 1 mM EDTA, pH 8.0, for 10-20 min followed by cooling at RT for 20 min. Antibody should be titrated for optimal results in individual systems.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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