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PC317L

Sigma-Aldrich

Anti-Angiogenin Goat pAb

lyophilized, Calbiochem®

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About This Item

UNSPSC Code:
12352203

biological source

goat

Quality Level

100

antibody product type

primary antibodies

clone

polyclonal

form

lyophilized

does not contain

preservative

species reactivity

human

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

isotype

IgG

shipped in

ambient

storage temp.

−20°C

General description

Anti-Angiogenin, goat polyclonal, recognizes angiogenin and can neutralize the activity of human recombinant angiogenin. It is validated for ELISA, Western blotting, and neutralization studies.
Immunoaffinity purified goat polyclonal antibody. Recognizes the ~14 kDa angiogenin protein.
Recognizes angiogenin.
  • Antibody Target Gene Symbol: ANG
  • Target Synonym: AI385586, ALS9, Ang1, Ang2, ANGIOGENIN, ANGIOGENIN 2, BRB, BRN, HEL168, MGC140149, MGC22466, MGC71966, RNASE4, RNASE5, Rnase5a
  • Entrez Gene Name: angiogenin, ribonuclease, RNase A family, 5
  • Hu Entrez ID: 283
  • Mu Entrez ID: 11727
  • Rat Entrez ID: 305843, 497229

    • Immunogen

    Human
    recombinant, human angiogenin

    Application

    ELISA (0.5-1 µg/ml)

    Immunoblotting (1-2 µg/ml)

    Neutralization Studies (EC₅₀= 100-150 µg/µg angiogenin, see comments)

    Physical form

    Lyophilized from 20 mM ammonium bicarbonate, PBS, 100 µg FAF-BSA.

    Preparation Note

    Reconstitute the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 1 mg/ml. Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C. Avoid freeze/thaw cycles of solutions.

    Analysis Note

    Positive Control
    HT-29 cells

    Other Notes

    Shapiro, R. 1998. Biochemistry37, 6847.
    Soncin, F., et al. 1997. Biochem. Biophys. Res. Commun.236 604.
    Vallee, B.L. and Riordan, J.F. 1997. Cell Mol. Life Sci.53 803.
    Moenner, M., et al. 1994. Eur. J. Biochem.226 483.
    Hu, G.F., et al. 1993. PNAS.90 1217.
    Moroianu, J., et al. 1993. PNAS.90 3815.
    Soncin, F. 1992. PNAS.89 2232.
    Lee, F.S. and Vallee, B.L. 1989. Biochem. Biophys. Res. Commun.161 121.
    Kurachi, K., et al. 1985. Biochemistry24, 5494.
    This antibody has been selected for its ability to neutralize the biological activity of human recombinant angiogenin. The activity of human recombinant angiogenin was measured based upon its ribonucleolytic activity toward yeast tRNA. Using the assay conditions described, 1 µg human recombinant angiogenin produces an absorbance change at 260 nm of ~2.0-3.0. The suggested antibody neutralization concentration required to yield 50% of the activity due to 1 µg of human recombinant angiogenin is approximately 100-150 µg of the antibody. The detection limit for human recombinant angiogenin by immunoblotting is ~5 ng/lane under reducing and non-reducing conditions. The detection limit for human recombinant angiogenin by ELISA is ~0.3 ng/well. This antibody exhibits no cross-reactivity with other cytokines when tested in ELISA. Antibody should be titrated for optimal results in individual systems.

    Legal Information

    CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

    Disclaimer

    Toxicity: Standard Handling (A)

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