Anti-Heparin Binding Epidermal Growth Factor (Ab-1) Goat pAb

Anti-Heparin Binding Epidermal Growth Factor (Ab-1), goat polyclonal, recognizes the ~19-23 kDa HB-EGF in vascular smooth muscle cells. It is validated for use in ELISA, WB & neutralization studies.

Purified goat polyclonal antibody. Recognizes the ~19-23 kDa (apparent MW) heparin binding epidermal growth factor protein.

Recognizes the ~19-23 kDa HB-EGF protein in vascular smooth muscle cells.

purified, recombinant, human HB-EGF

ELISA (0.5-1 µg/ml, see comments)

Immunoblotting (1-2 µg/ml)

Neutralization Studies (see comments)

Lyophilized from sterile filtered solution in PBS, 5% trehalose, 100 µg BSA.

Reconstitute the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 0.25 mg/ml. Lyophilized antibody should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C. Avoid freeze/thaw cycles of solutions.

Positive Control
Vascular smooth muscle cells

Davis-Fleischer, K.M. et al. 1998. Front. Biosci.3, D288.
Ouchi, N., et al. 1997. Biochem. J.328, 923.
Raab, G. and Klagsbrun M. 1997. Biochim. Biophys. Acta1333, F179.
Suzuki, M., et al. 1997. J. Biol. Chem.272, 31730.
Nakata, A., et al. 1996. Circulation94, 2778.
Miyagawa, J., et al. 1995. J. Clin. Invest.95, 404.

This antibody has been selected for its ability to neutralize the biological activity of human recombinant HB-EGF. It will not neutralize the biological activity of EGF, TGF-α or amphiregulin. The exact concentration of antibody required to neutralize human recombinant HB-EGF activity is dependent on the cytokine concentration, cell type, growth conditions, and the type of activity studied. Suggested neutralization concentration required to yield one-half maximal inhibition of HB-EGF activity is ~3-6 µg/ml in the presence of 10 ng/ml of human recombinant HB-EGF using a Balb/3T3 cell line. For immunoblotting, the detection limit for human recombinant HB-EGF is ~0.5 ng/lane under non-reducing and reducing conditions. For ELISA, the detection limit for human recombinant HB-EGF is ~0.03 ng/well. This antibody exhibits no cross-reactivity with other cytokines when tested in ELISA. Antibody should be titrated for optimal results in individual systems.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Toxicity: Harmful (C)