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PC344

Sigma-Aldrich

Anti-Netrin-1 (Ab-1) (557-568) Rabbit pAb

liquid, Calbiochem®

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About This Item

UNSPSC Code:
12352203

biological source

rabbit

Quality Level

antibody product type

primary antibodies

clone

polyclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

mouse

manufacturer/tradename

Calbiochem®

storage condition

do not freeze

isotype

IgG

shipped in

wet ice

storage temp.

2-8°C

General description

Purified rabbit polyclonal antibody. Recognizes the ~60 kDa netrin-1 protein.
Recognizes the ~70 kDa netrin-1 protein in embryonic mouse brain tissue.

Immunogen

Mouse
a synthetic peptide (LGNAEDSPDQSG) corresponding to amino acids 557-568 of mouse netrin-1, conjugated to KLH

Application


Immunoblotting (1 g/ml)
Frozen Sections (not recommended)

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In 0.05 M sodium phosphate buffer, 0.2% gelatin, pH 7.5.

Analysis Note

Positive Control
Mouse brain tissue

Other Notes

Immunoblotting was performed using brain tissue from various developmental stages. Netrin-1 protein is enriched in embryonic neural tissue. Antibody should be titrated for optimal results in individual systems. For immunohistochemistry applications, Anti-Netrin-1 (Ab-2) Rabbit pAb is also available (Cat. No. PC364-100 µg).
Varela-Echavarria, A., and Guthrie, S. 1997. Genes and Dev.11, 545.
Harris, R., et al. 1996. Neuron17, 217.
Keino-Masu, K., et al. 1996. Cell87, 175.
Mitchell, K.J., et al. 1996. Neuron17, 203.
Serafini, T., et al. 1996. Cell87, 1001.
Tessier-Lavigne, M. and Goodman, C.S. 1996. Science274, 1123.
Kennedy, T.E., et al. 1994. Cell78, 425.
Serafini, T., et al. 1994. Cell78, 409.
Ishii, N., et al. 1992. Neuron9, 873.
Hedgecock, E.M., et al. 1990. Neuron4, 61.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

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