Key Documents
PC362
Anti-Orexin A (14-33) (Ab-2) Rabbit pAb
liquid, Calbiochem®
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About This Item
UNSPSC Code:
12352203
biological source
rabbit
Quality Level
antibody product type
primary antibodies
clone
polyclonal
form
liquid
contains
≤0.1% sodium azide as preservative (antibody only)
species reactivity
mouse, human, rat, bovine
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
avoid repeated freeze/thaw cycles
isotype
IgG
shipped in
wet ice
storage temp.
−20°C
General description
Rabbit polyclonal antibody supplied as undiluted serum. Recognizes orexin A. Supplied with a control peptide.
This product has been discontinued.
Recognizes orexin A in mouse and rat brain. Supplied with a control peptide.
Recognizes orexin A in mouse and rat brain. Supplied with a control peptide.
Immunogen
Human
a synthetic peptide (CRLYELLHGAGNHAAGILTL) corresponding to amino acids 14-33 of human orexin A
Application
ELISA (see comments)
Free-floating Sections (1:3500)
Frozen Sections (1:3500)
Immunofluorescence (1:2000)
Physical form
Serum or ascites diluted in 5 mM phosphate buffer, pH 7.5 and 25 µg lyophilized control.
Preparation Note
Following initial thaw of antibody, aliquot and freeze (-20°C). Reconstitute control peptide with 25 µl dH₂O for a working stock of 1 mg/ml. Following reconstitution, aliquot and freeze (-20°C).
Analysis Note
Positive Control
Mouse or rat brain
Mouse or rat brain
Other Notes
De Lecea, L., et al. 1998. Proc. Natl. Acad. Sci. USA95, 322.
Flier, J.S. and Maratos-Flier, E. 1998. Cell92, 437.
Sakurai, T., et al. 1998. Cell92, 573.
Schwartz, M.W. 1998. Nat. Med.4, 385.
Flier, J.S. and Maratos-Flier, E. 1998. Cell92, 437.
Sakurai, T., et al. 1998. Cell92, 573.
Schwartz, M.W. 1998. Nat. Med.4, 385.
Immunohistochemistry was performed using floating mouse and rat brain sections fixed in 4% paraformaldehyde. Staining was completely abolished by preincubating the antiserum with control peptide at 10-6M. Staining is observed in the lateral hyothalamus. Unconjugated Orexin A Control Peptide has been included for use as an internal control for antibody specificity. This antibody has been tested to work in ELISA. Also available in purified form, Anti-Orexin A (14-33) (Ab-1) Rabbit pAb, Cat. No. PC345. Antibody should be titrated for optimal results in individual systems.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
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