Anti-p19 ^ARF (54-75) (Ab-1) Rabbit pAb

Immunoaffinity purified rabbit polyclonal antibody. Recognizes the ~19 kDa p19^ARF protein.

In humans, the multiple tumor suppressor 1 locus (MTS1) encodes two unrelated genes, p16^INK4a and p19^ARF. Although both act as cell proliferation inhibitors, their mechanisms of action are different. p19^ARF works as part of a p53-dependant pathway to counter uncontrolled proliferation and oncogenic signals. Mice lacking the p19^ARF gene rapidly develop a broad spectrum of tumors. This result indicates that p19^ARF is an important tumor suppressor. In addition, p19^ARF helps prevent transforming signals from various oncoproteins via the p53 regulatory loop. These studies and others display the importance of p19^ARF in cell cycle control and tumor suppression. p19^ARF recognizes in vitro translated murine p19^ARF and a ~19 kDa band in murine CTLL2 cells.

Recognizes the ~19 kDa p19^ARF protein in CTLL2 cells and mouse embryo fibroblasts.

This Anti-p19^ARF (54-75) (Ab-1) Rabbit pAb is validated for use in Immunoblotting for the detection of p19^ARF (54-75) (Ab-1).

a synthetic peptide corresponding to amino acids 54-75 of mouse p19^ARF, conjugated to KLH

Immunoblotting (0.5-1 μg/ml)

In PBS, 1% BSA.

Following initial thaw, aliquot and freeze (-20°C).

Positive Control
Mouse embryo fibroblasts or CTLL2 cells

Antibody should be titrated for optimal results in individual systems.