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PC450

Sigma-Aldrich

PhosphoDetect Anti-Tie2 (pTyr 1108/1117) (Ab-2) Rabbit pAb

liquid, Calbiochem®

Synonym(s):

Anti-Angiopoietin Receptor, Anti-Tek

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About This Item

UNSPSC Code:
12352203

biological source

rabbit

Quality Level

antibody product type

primary antibodies

clone

polyclonal

form

liquid

does not contain

preservative

species reactivity

human

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

isotype

IgG

shipped in

wet ice

storage temp.

−20°C

General description

Rabbit polyclonal antibody supplied as undiluted serum. Recognizes the ~140-145 Tie2 protein phosphorylated at Tyr1108 and Tyr1117.
This product has been discontinued.

Recognizes the ~140-145 Tie2 protein phosphorylated at Tyr1108 and Tyr1117 in colon carcinoma tissue.

Immunogen

a synthetic phosphopeptide [pYEKFTpYAGID(C)] corresponding to amino acids surrounding the Tyr1108/1117 phosphorylation sites 1108-1117 of human Tie2

Application


ELISA (see comments)
Paraffin Sections (1:2000-1:5000, no pre-treatment required)

Analysis Note

Positive Control
Colon carcinoma tissue

Other Notes

Hayes, A.J., et al. 1999. Microvasc. Res.58, 2224.
Jones, N., et al. 1999. J. Biol. Chem.274, 30896.
Valenzuela, D.M., et al. 1999. Proc. Natl. Acad. Sci. USA96, 1904.
Jones, N. and Dumont, D.J. 1998. Oncogene17, 1097.
This antibody can be used in conjunction with a biotinylated phosphopeptide to measure the level of Tie2 activity by competition ELISA. Antibody should be titrated for optimal results in individual systems.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

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