PC69
Anti-Fas (Ab-1) (321-335) Rabbit pAb
liquid, Calbiochem®
Synonym(s):
Anti-APO-1, Anti-CD95
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About This Item
UNSPSC Code:
12352203
biological source
rabbit
Quality Level
antibody product type
primary antibodies
clone
polyclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity
opossum, rat, human, mouse
manufacturer/tradename
Calbiochem®
storage condition
do not freeze
isotype
IgG
shipped in
wet ice
storage temp.
2-8°C
General description
Anti-Fas (Ab-1) (321-335), rabbit polyclonal, recognizes the ~44-46 kDa Fas in HL60 cells. It is validated for use in WB and IHC (frozen and paraffin sections).
Purified rabbit polyclonal antibody. Recognizes the ~44-46 kDa Fas protein.
Recognizes the ~44-46 kDa Fas protein in HL60 cells and color tissue.
The immunogen, Fas Peptide (Cat. No. PP62), is available for competition studies.
Immunogen
Human
a synthetic peptide (DSENSNFRNEIQSLV) corresponding to amino acids 321-335 of human Fas
Application
Frozen Sections (2.5 µg/ml)
Immunoblotting (2.5 µg/ml)
Paraffin Sections (2.5 µg/ml, pressure cooker pre-treatment required)
Immunoblotting (2.5 µg/ml)
Paraffin Sections (2.5 µg/ml, pressure cooker pre-treatment required)
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In 0.05 M sodium phosphate buffer, 0.2% gelatin.
Analysis Note
Positive Control
HL-60 or colon tissue
HL-60 or colon tissue
Other Notes
Cheng, J., et al. 1995. J. Immunol.154, 1239.
Stanger, B.Z., et al. 1995. Cell81, 513.
Grell, M., et al. 1994. Eur. J. Immunol.24, 2563.
Owen-Schaub, L.B., et al. 1994. Cancer Res.54, 1580.
Itoh, N. and Nagata, S. 1993. J. Biol. Chem.268, 10932.
Oehm, A., et al. 1992. J. Biol. Chem.267, 10709.
Watanabe-Fukunaga, R., et al. 1992. J. Immunol.148, 1274.
Itoh, N., et al. 1991. Cell66, 233.
Stanger, B.Z., et al. 1995. Cell81, 513.
Grell, M., et al. 1994. Eur. J. Immunol.24, 2563.
Owen-Schaub, L.B., et al. 1994. Cancer Res.54, 1580.
Itoh, N. and Nagata, S. 1993. J. Biol. Chem.268, 10932.
Oehm, A., et al. 1992. J. Biol. Chem.267, 10709.
Watanabe-Fukunaga, R., et al. 1992. J. Immunol.148, 1274.
Itoh, N., et al. 1991. Cell66, 233.
Will detect Fas using frozen sections but not immunoblots of mouse and rat samples. Antibody should be titrated for optimal results in individual systems.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
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Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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