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PH02

Sigma-Aldrich

Anti-PTP1B (Ab-2) Mouse mAb (AE4-2J)

liquid, clone AE4-2J, Calbiochem®

Synonym(s):

Anti-Protein Tyrosine Phosphatase 1B

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About This Item

UNSPSC Code:
12352203

biological source

mouse

antibody product type

primary antibodies

clone

AE4-2J, monoclonal

form

liquid

does not contain

preservative

species reactivity

human

should not react with

mouse

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

isotype

IgG1

shipped in

wet ice

storage temp.

−20°C

General description

This product has been discontinued.



Recognizes the ~50 kDa PTPase 1B protein.

Mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with SP2/0 myeloma cells. Recognizes the ~50 kDa full-length PTPase 1B protein.

Immunogen

Epitope: within the C-terminal 120 amino acids
Human
recombinant, human protein tyrosine phosphatase 1B (PTPase 1B)

Application

Immunoblotting (1-5 µg/ml)

Immunofluorescence (1-5 µg/ml)

Immunoprecipitation (1-2 µg/sample)

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In 0.05 M sodium phosphate buffer, 50% glycerol.

Preparation Note

Following initial thaw, aliquot and freeze (-20°C).

Analysis Note

Positive Control
HT-1080 or SW480 cells

Other Notes

Fischer, E.H., et al. 1991. Science253, 401.
Pallen, C.J., et al. 1991. Biochem. J.276, 315.
Brown-Shimer, S., et al. 1990. Proc. Natl. Acad. Sci. USA87, 5148.
Chernoff, J., et al. 1990. Proc. Natl. Acad. Sci. USA87, 2735.
Charbonneau, H., et al. 1989. Proc. Natl. Acad. Sci. USA86, 5252.
Charbonneau, H., et al. 1988. Proc. Natl. Acad. Sci. USA85, 7182.
Recognizes only full-length PTPase 1B. May also detect a 90-95 kDa protein by immunoblotting (but not immunoprecipitation) that may be mor intense than PTPase 1B (see application references). Antibody should be titrated for optimal results in individual sample types.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

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