PH03L
Anti-PTP, T Cell (Ab-1) Mouse mAb (CF4-1D)
lyophilized, clone CF4-1D, Calbiochem®
Synonym(s):
Anti-Protein Tyrosine Phosphatase, T cell
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About This Item
UNSPSC Code:
12352203
biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
CF4-1D, monoclonal
form
lyophilized
does not contain
preservative
species reactivity
human
should not react with
mouse
manufacturer/tradename
Calbiochem®
storage condition
do not freeze
isotype
IgG2a
shipped in
ambient
storage temp.
2-8°C
General description
Purified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with SP2/0 mouse myeloma cells. Recognizes the PTPase protein.
Recognizes the T-cell PTPase protein.
This Anti-PTP, T Cell (Ab-1) Mouse mAb (CF4-1D) is validated for use in Immunocytochemistry, Immunoblotting, Immunoprecipitation for the detection of PTP, T Cell (Ab-1).
Immunogen
Human
recombinant, human T cell PTPase
Application
Immunocytochemistry (see comments)
Immunoblotting (1-5 µg/ml)
Immunoprecipitation (1-2 µg/sample)
Immunoblotting (1-5 µg/ml)
Immunoprecipitation (1-2 µg/sample)
Physical form
Lyophilized from 20 mM sodium bicarbonate, 100 µg BSA.
Preparation Note
Reconstitute the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4 to yield a final concentration of 100 µg/ml. Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Following reconstitution, aliquot and freeze (-20°C) for long-term storage or refrigerate (4°C) with 0.1% azide for short-term storage. Avoid freeze/thaw cycles of solutions.
Analysis Note
Positive Control
CCRF-CEM cells
CCRF-CEM cells
Other Notes
Brown-Shimer, S., et al. 1992. Can. Res.52, 478.
Fischer, E.H., et al. 1991 Science253, 401.
Brown-Shimer, S., et al. Proc. Natl. Acad. Sci. USA87, 5148.
Chernoff, J., et al. 1990. Proc. Natl. Acad. Sci. USA87, 2735.
Cool, D.E., et al. 1990. Proc. Natl. Acad. Sci. USA87, 7280.
Charbonneau, H., et al. 1989. Proc. Natl. Acad. Sci. USA86, 5252.
Cool, D.E., et al. 1989. Proc. Natl. Acad. Sci. USA86, 5257.
Charbonneau, H., et al. 1988. Proc. Natl. Acad. Sci. USA85, 7182.
Fischer, E.H., et al. 1991 Science253, 401.
Brown-Shimer, S., et al. Proc. Natl. Acad. Sci. USA87, 5148.
Chernoff, J., et al. 1990. Proc. Natl. Acad. Sci. USA87, 2735.
Cool, D.E., et al. 1990. Proc. Natl. Acad. Sci. USA87, 7280.
Charbonneau, H., et al. 1989. Proc. Natl. Acad. Sci. USA86, 5252.
Cool, D.E., et al. 1989. Proc. Natl. Acad. Sci. USA86, 5257.
Charbonneau, H., et al. 1988. Proc. Natl. Acad. Sci. USA85, 7182.
This antibody has been reported to work for immunocytochemistry. Antibody should be titrated for optimal results in individual systems.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
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Storage Class Code
10-13 - German Storage Class 10 to 13
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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