SCR128
Dopaminergic Differentiation Growth Factor Sampler
This Dopaminergic Differentiation Growth Factor Sampler contains five validated growth factors used to induce differentiation of human pluripotent Embryonic stem (ES) & induced pluripotent Stem (iPS) cells to dopaminergic neurons.
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About This Item
Product Name
Dopaminergic Differentiation Growth Factor Sampler, This Dopaminergic Differentiation Growth Factor Sampler contains five validated growth factors used to induce differentiation of human pluripotent Embryonic stem (ES) & induced pluripotent Stem (iPS) cells to dopaminergic neurons.
assay
>98% (SDS-PAGE and HPLC)
impurities
<0.1 ng/μg endotoxin (1EU/μg)
shipped in
dry ice
Quality Level
Analysis Note
Application
Stem Cell Research
Growth Factors & Receptors
Biochem/physiol Actions
None
Disclaimer
General description
Other Notes
2. Fibroblast Growth Factor-8 (FGF-8), Human Recombinant (Part No. GF110-5UG): One 5 μg vial. Lyophilized powder. Store at -20°C.
3. Brain Derived Neurotrophic Factor (BDNF), Human Recombinant (Part No. GF029-2UG): One 2 μg vial. Lyophilized powder. Store at -20°C.
4. Glial Derived Neurotrophic Factor (GDNF), Human Recombinant (Part No. GF030-2UG): One 2 μg vial. Lyophilized powder. Store at -20°C.
5. Transforming Growth Factor β-III (TGF-β-III), Human Recombinant (Part No. GF176-2UG): One 2 μg vial. Lyophilized powder. Store at -20°C.
6. Anti-Tyrosine Hydroxylase antibody, rabbit polyclonal (Part No. AB152-20): One 20 μL vial. Store at -20°C.
Preparation Note
signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2
Storage Class
10 - Combustible liquids
Certificates of Analysis (COA)
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Articles
Human iPSC neural differentiation media and protocols used to generate neural stem cells, neurons and glial cell types.
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Dual SMAD inhibition is a well-established method to derive neural progenitor cells from both human ES and iPS cells. This protocol uses two SMAD inhibitors, Noggin and SB431542, to drive the rapid differentiation of ES/iPS cells into a highly enriched population of NPCs. Noggin acts as a BMP inhibitor and SB431542 inhibits the Lefty/Activin/TGFβ pathways by blocking the phosphorylation of ALK4, ALK5, and ALK7 receptors. In an effort to make a more defined and optimized neuronal differentiation protocol, Li and colleagues modified the original protocol to establish a completely small molecule-based differentiation method, which relies on three small molecules to inhibit GSK-3β (CHIR99021), TGFβ (SB431542), and Notch (compound E) signaling pathways, along with human LIF3. This new small molecule-based neural differentiation protocol increased neural differentiation kinetics and allowed the derivation of truly multipotent neural stem cells that respond to regional patterning cues specifying forebrain, midbrain, and hindbrain neural and glial subtypes.
Global Trade Item Number
| SKU | GTIN |
|---|---|
| SCR128 | 04053252551215 |
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