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YEAST1

Yeast Transformation Kit

reagents for introducing plasmid DNA into yeast

Synonym(s):

lithium acetate yeast transformation

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About This Item

NACRES:
NA.85
UNSPSC Code:
12352200

Product Name

Yeast Transformation Kit, reagents for introducing plasmid DNA into yeast

grade

Molecular Biology

technique(s)

transformation: suitable

shipped in

dry ice

storage temp.

−20°C

Quality Level

200

usage

 kit sufficient for >100 standard transformations

Related Categories

Application

Suitable for transformation of any strain of yeast. Convenient, flexible and sensitive, positive transformants can be obtained with as little as 10 ng of DNA; the optimum efficiency is in the 0.1- 3 μg range.

Biochem/physiol Actions

Transformation with a plasmid complementing the mutated gene enables the transformant to grow on medium lacking the required component. Yeast cells are made competent for transformation by incubation in a buffered lithium acetate solution. Transformation is then carried out by incubating the cells together with transforming DNA and carrier DNA in a solution containing polyethylene glycol (PEG).

Features and Benefits

  • Easy and ready-to-use
  • Requires as little as 10 ng of plasmid DNA
  • Flexibility for any strain of yeast
  • Sufficient for over 100 standard transformations

General description

Sigma′s Yeast Transformation Kit contains all necessary reagents and controls for efficient transformation of yeast by the lithium acetate method.

Other Notes

The Yeast Transformation Kit contains:
  • Transformation Buffer; 100 ml; 100 mM lithium acetate, 10 mM Tris HCl, pH 7.6, and 1 mM EDTA
  • Plate Buffer; 100 ml; 40% PEG, 100 mM lithium acetate, 10 mM Tris HCl, pH 7.5, 1 mM EDTA
  • Deoxyribonucleic acid from salmon teste, 10 mg/ml; 2 x 1 ml
  • Control Yeast Plasmid DNA pRS316 carrying the ura gene; 10 μg
  • Yeast Synthetic Drop-out Medium Supplement Without Uracil; 1 g

Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
0000370527
0000359480
0000370527
0000359480
0000228907

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A simple and efficient procedure for transformation of yeasts.
R Elble
BioTechniques, 13(1), 18-20 (1992-07-01)
Improved method for high efficiency transformation of intact yeast cells.
D Gietz et al.
Nucleic acids research, 20(6), 1425-1425 (1992-03-25)
Tim R Blower et al.
Nucleic acids research, 47(15), 8163-8179 (2019-07-10)
Type II topoisomerases catalyze essential DNA transactions and are proven drug targets. Drug discrimination by prokaryotic and eukaryotic topoisomerases is vital to therapeutic utility, but is poorly understood. We developed a next-generation sequencing (NGS) approach to identify drug-resistance mutations in
Widening the pH activity profile of a fungal laccase by directed evolution.
Torres-Salas P
Chembiochem, 14(8), 934-937 (2013)
Miren Zumárraga et al.
Chemistry & biology, 14(9), 1052-1064 (2007-09-22)
Fungal laccases are remarkable green catalysts that have a broad substrate specificity and many potential applications in bioremediation, lignocellulose processing, organic synthesis, and more. However, most of these transformations must be carried out at high concentrations of organic cosolvents in
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Articles

Protein Expression Systems

Genetic engineering enables large-scale expression and isolation of recombinant proteins for research purposes.

Yeast Transformation Introduction

Transformation introduces exogenous DNA into cells, a fundamental genetic modification process demonstrated in Streptococcus pneumoniae.

Protocols

Yeast Transformation Protocols

Yeasts are considered model systems for eukaryotic studies as they exhibit fast growth and have dispersed cells.

Yeast Transformation Kit

The selection of plasmids in yeast is based on the use of auxotrophic mutant strains, which cannot grow without a specific medium component (an amino acid, purine, or pyrimidine)

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