SAE0044

Choline Oxidase from Arthrobacter sp.

Name: Choline Oxidase from <I>Arthrobacter</I> sp.
Brand: SIGMA

recombinant, expressed in E. coli ≥12 units/mg protein

Synonym(s):

COD, COX, ChOx, Choline: oxygen 1-oxidoreductase

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All Photos(4)

About This Item

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

1.1.3.17 (BRENDA, IUBMB)

Product Name

Choline Oxidase from Arthrobacter sp., recombinant, expressed in E. coli ≥12 units/mg protein

biological source

bacterial (arthrobacter sp.)

recombinant

expressed in E. coli ≥12 units/mg protein

form

powder

specific activity

≥12 units/mg protein

technique(s)

activity assay: suitable

UniProt accession no.

A0A0Q9NRQ3
A0A0Q9PH20

storage temp.

−20°C

Quality Level

200

Application

Choline oxidase from Arthrobacter sp. has been used in the enzymatic determination of choline in milk using a flow injection analysis (FIA) system with potentiometric detection. It has been used for fluorescent detection of choline released during autotaxin (ATX) activity in conditioned media and plasma.

Biochem/physiol Actions

Choline oxidase from Arthrobacter sp. is a flavoprotein, which is a member of the GMC-oxidoreductase family. The enzyme catalyzes the four-electron-oxidation of choline to glycine betaine via the intermediate betaine aldehyde, in two sequential FAD-dependent reaction steps. The enzyme is useful for enzymatic determination of phosphatidylcholine by coupling with phospholipase D or determination of sphingomyelin by coupling with sphingomyelinase and for cholinesterase activity assays.Choline oxidase (COD) mediating glycine betaine biosynthetic pathway plays a vital role in creating stress resistant transgenic plants.

Preparation Note

It is recommended to reconstitute choline oxidase in phosphate buffer at pH 7.5 supplemented with 10mM EDTA. Reconstituted material can be stored at 2-8 °C for at least 2 weeks.

Tightly closed.Dry. Keep locked up or in an area accessible only to qualified or authorized
persons

General description

Research area: Cell Signaling

Choline oxidase from Arthrobacter sp. is a flavoprotein, which is a member of the glucose–methanol–choline (GMC). The codA gene encodes the choline oxidase enzyme.

Other Notes

One unit will form 1 μmole of H₂O₂ with oxidation of 1 μmole of choline to betaine aldehyde per min at pH 8.0 at 37 °C. Note: During the conversion of choline to betaine by choline oxidase, 2 μmoles of H₂O₂ are produced for every μmole of choline.

pictograms

GHS08

signalword

Danger

hcodes

H334

pcodes

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Transformation of Arabidopsis thaliana with the codA gene for choline oxidase; accumulation of glycinebetaine and enhanced tolerance to salt and cold stress.

Hayashi H, et al.

The Plant Journal, 12(1), 133-142 (1997)

Transformation of Synechococcus with a gene for choline oxidase enhances tolerance to salt stress.

Deshnium P, et al.

Plant Molecular Biology, 29(5), 897-907 (1995)

Rapid, specific, and sensitive measurements of plasma sphingomyelin and phosphatidylcholine.

Hojjati M.R., and Jiang X.C.

Journal of Lipid Research, 47, 673-676 (2006)

New enzymatic assay of cholinesterase activity

Okabe, H. et al.,

Clinica Chimica Acta; International Journal of Clinical Chemistry, 80, 87-94 (1977)

The use of bacterial choline oxidase, a glycinebetaine-synthesizing enzyme, to create stress-resistant transgenic plants

Plant Physiology, 180-188 (2001)

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