T7451

Anti-Acetylated Tubulin antibody, Mouse monoclonal

Name: Anti-Acetylated Tubulin antibody, Mouse monoclonal
Brand: SIGMA

clone 6-11B-1, purified from hybridoma cell culture

Synonym(s):

Acetylated Tubulin Antibody, Acetylated Tubulin Antibody - Monoclonal Anti-Acetylated Tubulin antibody produced in mouse, Anti Acetyl Tubulin antibody, Anti Acetyl-alpha tubulin

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About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

MDL number:

MFCD00164512

Product Name

Anti-Acetylated Tubulin antibody, Mouse monoclonal, clone 6-11B-1, purified from hybridoma cell culture

biological source

mouse

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

6-11B-1, monoclonal

form

buffered aqueous solution

species reactivity

bovine, frog, invertebrates, human, hamster, mouse, protista, pig, monkey, chicken, rat, plant

packaging

antibody small pack of 25 μL

concentration

~1 mg/mL

technique(s)

dot blot: suitable
electron microscopy: suitable
immunohistochemistry: suitable
radioimmunoassay: suitable
western blot: 0.03-0.06 μg/mL using total rat brain extract

isotype

IgG2b

UniProt accession no.

P68366

application(s)

research pathology

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

acetylation (Lys40)

Immunogen

acetylated tubulin from the outer arm of Strongylocentrotus purpuratus (sea urchin).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Application

Monoclonal Anti-Acetylated Tubulin antibody produced in mouse has been used in:

quantitative dot blot
immunofluorescence
Western blot
immunocytochemistry
enzyme-linked immunosorbent assay (ELISA)
solid-phase radioimmunoassay (RIA)
electron microscopy

Anti-acetylated tubulin antibody has also been used in staining X-gal–stained paraffin sections to perform immunohistochemistry.

Biochem/physiol Actions

Acetylated tubulin is associated with axoneme assembly in a variety of organisms. Acetylated tubulin might play a crucial role in the differentiation of microtubule structure and function. Post translational modification of α-tubulin i.e. the reversible addition of an acetyl group to Lys40, by specific acetylase, results in acetylated tubulin.

The antibody has been used to detect acetylated α-tubulins from many organisms including protista, plants, invertebrates, and vertebrates. The antibody recognizes an epitope located on the α3 isoform of Chlamydomonas axonemal α-tubulin, within four residues of Lys⁴⁰ when this amino acid is acetylated. A sequence very similar to the one detected by the antibody in Chlamydomonas is found in the majority of α-tubulins, but the corresponding region is markedly divergent in some α-tubulin isoforms from chicken, Drosophila, and yeast.Occasionally, the epitope recognized by the antibody may be absent or masked, as it is in the rat kangaroo epithelial-like cell line PtK₂.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

α-tubulin, also called as tubulin α 4a (TUBA4A), is mapped to human chromosome 2q35. The gene codes for a member of the α -tubulin family containing 448 amino acids. TUBA4A belongs to the subfamily of tubulin, which is the major building block of microtubules. α-subunit of tubulin has molecular weight of 50,000.

Monoclonal anti-acetylated tubulin antibody (mouse IgG2b isotype) is derived from the hybridoma 6-11B-1 produced by the fusion of mouse myeloma cells and splenocytes from a mouse immunized with acetylated tubulin from the outer arm of Strongylocentrotus purpuratus (sea urchin). Tubulin is a heterodimer, which consists of a-tubulin and b-tubulin; both subunits have a molecular weight of 50,000 and share considerable homology.

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Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Certificates of Analysis (COA)

Lot/Batch Number

0000445901

0000445900

0000398828

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Heterozygous loss-of-function SEC61A1 mutations cause autosomal-dominant tubulo-interstitial and glomerulocystic kidney disease with anemia

Bolar NA, et al.

American Journal of Human Genetics, 99(1), 174-187 (2016)

Inhibition of histone deacetylases 1 and 6 enhances cytarabine-induced apoptosis in pediatric acute myeloid leukemia cells.

Xu X

PLoS ONE, 6(2) (2011)

Basal cells as stem cells of the mouse trachea and human airway epithelium.

Rock JR

Proceedings of the National Academy of Sciences of the USA, 106(31), 12771-12775 (2009)

Loss-of-Function GAS8 Mutations Cause Primary Ciliary Dyskinesia and Disrupt the Nexin-Dynein Regulatory Complex.

Heike Olbrich et al.

American journal of human genetics, 97(4), 546-554 (2015-09-22)

Multiciliated epithelial cells protect the upper and lower airways from chronic bacterial infections by moving mucus and debris outward. Congenital disorders of ciliary beating, referred to as primary ciliary dyskinesia (PCD), are characterized by deficient mucociliary clearance and severe, recurrent

Acetylation: a regulatory modification to rival phosphorylation?

Kouzarides T

The Embo Journal, 19, 1176-1179 (2000)

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