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cOmplete™ His-Tag Purification Resin Protocol & Troubleshooting

General guidance for the detection and enumeration of Enterobacteriaceae in food according to EN-ISO 8523:1991 and EN-ISO 4832:1991, respectively.

CRISPR Cas 9 Nuclease RNA-guided Genome Editing

Learn about CRISPR Cas9, what it is and how it works. CRISPR is a new, affordable genome editing tool enabling access to genome editing for all.

Enzymatic Assay of Esterase

Objective: To standardize a procedure for the enzymatic determination of Esterase activity using Ethyl Butyrate as a substrate.

Enzymatic Assay of Acid Phosphatase (EC 3.1.3.2)

Rapid DNA Ligation Kit Protocol

Procedure for Enzymatic Assay of α-Chymotrypsin (EC 3.4.21.1)

Follow our procedure for the determination of a chymotrypsin activity. This enzymatic assay of alpha chymotrypsin guides you through the entire process and necessary calculations.

MSTFA/MSTFA-d9 Derivatization of Amphetamine for GC/MS Detection and Identification

Example displays how MSTFA/MSTFA-d9 derivatization of amphetamine provides valuable information in mass spectra and chromatograms using SLB-5ms GC column.

Duolink® PLA Probemaker Guide

Guide to create custom PLA probes for immunofluorescent or brightfield detection.

Assay Procedure for Protease

This procedure is for informational purposes including assay Procedure, definition, and calculations for Protease

Determination of Water Content in Glycerine, Glycerol, 1,2,3-Propantriol Using Karl Fischer Titration

Summary application report for analysis of moisture in Glycerine, Glycerol, 1,2,3-Propantriol

Performing a Purification of Antibody Fragments with Capto™ L Products

This page shows how to purify antibody fragments by afﬁnity chromatography using Capto L products from Cytiva.

Performing a Purification of IgG Antibodies with Protein G HP SpinTrap™/Ab SpinTrap™

This page shows how to separate IgG antibodies by afﬁnity chromatography using Protein G HP SpinTrap™/Ab SpinTrap™ from Cytiva.

Performing a Purification of IgG Antibodies with Protein G Sepharose® 4 Fast Flow

This page shows how to separate IgG antibodies by afﬁnity chromatography using Protein G Sepharose 4 Fast Flow from Cytiva.

Nucleic Acid Sample Preparation Methodology

Spectrophotometry can be used to estimate DNA or RNA concentration and to analyze the purity of the preparation.

QuickLink™ DNA Ligation Kit

T4 DNA ligase is used for the joining of DNA molecules with compatible cohesive (sticky) termini, joining of blunt ended double stranded DNA molecules

SOURCE™: Purification at High Throughput with High Resolution and Easy Scale-Up

This page covers using SOURCE™ medias for the purification of proteins, peptides, or oligonucleotides.

Chromatofocusing: Principles and Methods

This page covers the principles and methods of chromatofocusing, a chromatography technique that separates proteins according to differences in their isoelectric point (pI).

Sepharose Big Beads: Purification from Crude, Viscous Samples at Large Scale

This page covers how and why to use Sepharose Big Beads for purification from crude, viscous samples.

pGEX Vectors

This page describes the characteristics of pGEX expression vectors used with Cytiva products.

LC/MS Analysis of L-Methionine Sulfoximine, Sulfone and Sulfoxide on Ascentis® Express HILIC

Separation of L-Methionine sulfone; L-Methionine sulfoximine; L-Methionine sulfoxide

Peptide Quick Tips

Peptide Quick Tips. Helpful guidelines for proper storage and resuspension of your peptide for optimal performance in your assay.

Converting From Linear Flow (cm/hour) to Volumetric Flow Rates (mL/min) and Vice Versa

This page shows how to convert between linear ﬂow and volumetric ﬂow rates in affinity chromatography.

Enzymatic Assay of Diamine Oxidase (E.C. No. 1.4.3.22)

To standardize a procedure for the enzymatic assay of Diamine Oxidase.

GC Analysis of Glycerides in Coconut Oil (Organic, Extra Virgin) on SLB-35ms

Separation of T30; T36; T42; T48

HPLC Analysis of LMW Heparin-Dalteparin and Enoxaparin Analysis on Zenix®-100

High resolution separations of low molecular weight heparin-daletparin and enoxaparin can be achived using Zenix SEC-100 columns.

Determination of Water Content in D(+)-lactose-monohydrate Using Karl Fischer Titration

Sepharose® High Performance: Purification with High Resolution

Here we discuss purification options when working with Sepharose® High Performance and we present some examples of media screening, capture and purification.

Conditioning Purified Membrane Proteins

This page shows how to condition membrane proteins for further analysis with products from Cytiva.

Purification using Glutathione Sepharose® High Performance, Glutathione Sepharose® 4 Fast Flow, and Glutathione Sepharose® 4B

This page shows how to purify GST-tagged proteins using Glutathione Sepharose from Cytiva.

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