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Characterization of human papillomavirus 6b L1 virus-like particles isolated from silkworms using capillary zone electrophoresis.

Characterization of human papillomavirus 6b L1 virus-like particles isolated from silkworms using capillary zone electrophoresis.

Journal of bioscience and bioengineering (2014-04-04)

Thomas Hahne, Muthukutty Palaniyandi, Tatsuya Kato, Peter Fleischmann, Hermann Wätzig, Enoch Y Park

ABSTRACT

Human papillomavirus 6b L1 virus-like particles (VLPs) were successfully expressed using Bombyx mori nucleopolyhedrovirus (BmNPV) bacmid expression system and rapidly purified using size exclusion chromatography after ultracentrifugation procedure and characterized by capillary zone electrophoresis (CZE). The average capillary electrophoresis migration time was 11 min with the relative standard deviation (RSD) of 0.3% of human papillomavirus 6b L1 VLPs. After this threefold fractionation, the CZE samples were still further investigated by dynamic light scattering and immuno blotting. The versatile technique, CZE not only proved to be a valuable tool for VLP characterization, but was also found to be reliable and precise. Thus CZE will also be an important option for the quality control of VLPs in pharmaceutical research level.

MATERIALS

Product Number

Brand

Product Description

Sodium chloride, random crystals, 99.9% trace metals basis

Sodium chloride-³⁵Cl, 99 atom % ³⁵Cl

Potassium chloride, AnhydroBeads^™, −10 mesh, 99.99% trace metals basis

Sodium chloride solution, 5 M

Supelco

Phosphate Standard for IC, TraceCERT^®, 1000 mg/L phosphate in water (nominal concentration)

Sodium hydroxide, reagent grade, ≥98%, pellets (anhydrous)

Sigma-Aldrich

Sodium phosphate dibasic solution, BioUltra, 0.5 M in H₂O

Sodium hydroxide solution, 50% in H₂O

Sodium hydroxide, BioUltra, suitable for luminescence, ≥98.0% (T), pellets

Sodium hydroxide solution, 49-51% in water, eluent for IC

Sodium hydroxide, pellets, semiconductor grade, 99.99% trace metals basis

Sodium hydroxide solution, purum, ≥32%

Sodium chloride, BioUltra, Molecular Biology, ≥99.5% (AT)

Sodium chloride, AnhydroBeads^™, −10 mesh, 99.999% trace metals basis

Sodium hydroxide solution, 5.0 M

Sodium hydroxide, reagent grade, 97%, flakes

Sodium hydroxide concentrate, 0.1 M NaOH in water (0.1N), Eluent concentrate for IC

Sodium hydroxide, ACS reagent, ≥97.0%, pellets

Sodium hydroxide solution, BioUltra, Molecular Biology, 10 M in H₂O

Sodium chloride solution, 5 M

Sodium chloride, BioPerformance Certified, ≥99% (titration), suitable for insect cell culture, suitable for plant cell culture

Sodium hydroxide, beads, 16-60 mesh, reagent grade, 97%

Sodium hydroxide, reagent grade, 97%, powder

Sodium hydroxide, puriss. p.a., ACS reagent, K ≤0.02%, ≥98.0% (T), pellets

Sodium hydroxide solution, 1.0 N, BioReagent, suitable for cell culture

Sodium hydroxide, puriss., meets analytical specification of Ph. Eur., BP, NF, E524, 98-100.5%, pellets

Sodium chloride solution, 5 M in H₂O, BioReagent, Molecular Biology, suitable for cell culture

Sodium hydroxide, BioXtra, ≥98% (acidimetric), pellets (anhydrous)

Sodium chloride, reference material for titrimetry, certified by BAM, >99.5%

Sodium chloride solution, 0.9% in water, BioXtra, suitable for cell culture