[Expression and action of keratinocyte growth factor, keratinocyte growth factor receptor on CaSki cell].

To investigate the expressions of keratinocyte growth factor (KGF), keratinocyte growth factor receptor (KGFR) in CaSki cell and action of keratinocyte growth factor on proliferation, migration of the CaSki cell. ELISA and Western blot methods were used to determine the protein expressions of the KGF and KGFR in CaSki cell respectively: 3H-Thymidine incorporation method was used to determine the effect of recombinant human KGF and anti-KGF on CaSki cell proliferation; Millicell-PCF was used to determine the effect of recombinant human KGF, anti-KGF on CaSki cell migration. Both KGF and KGFR were expressing in the CaSki cell; Recombinant human KGF resulted in a increase in the proliferation and migration of CaSki cells; The proliferation and migration of CaSki cell became weak due to autocrine KGF neutralized by KGF antibodies (P < 0.05). Current results demonstrate that KGF and KGFR express in CaSki cell; Both autocrine and recombinant human KGF have the effect on CaSki cell proliferation and migration.