Advanced Topics in Optimization, Design and Operation of Tangential Flow Filtration Processes
TRTFF04 Modules - One-day Course
This high level course explores the implementation of cutting-edge TFF techniques and technologies.
This course includes a choice of technically rigorous modules which address high-level scientific concerns within TFF design and implementation, including high-viscosity TFF (HVTFF), single-pass TFF design (SPTFF) and operation, and two-pump microfiltration (MFTFF).
To fit with your training needs, these courses are modular and can be selected individually or in combination with each other.
HVTFF Module: High-viscosity TFF
There is an increasing need for high concentration biopharmaceuticals. TFF is typically used to concentrate the drug substance, and this module addresses the many challenges associated with conducting very high-concentration endpoint TFF operations.
This module evaluates why high-concentration TFF processes are difficult to implement and how these challenges can be surmounted during process development.
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What will you be able to do after attending this course?
- Explain the hydrodynamics and rheology which influences pressure drops in TFF processes.
- Explain the challenges and strategies employed to successfully process high-viscosity products using TFF.
- Describe phenomena which can occur at high concentrations, such as the Donnan effect.
- Operate and optimize processes with highly concentrated material in the laboratory.
Course Program
Day 1: 9:00 – 17:30
- Welcome and course introduction
- HVTFF theory
- Processing challenges
- Practical operative recommendations
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- High protein concentration phenomena (Donnan effect)
- Practical session
- Assessment
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SPTFF Module: Single-pass TFF
Single-pass TFF has been proposed as an effective unit operation for concentration of product either continuously or for products where multiple pump passes are unacceptable.
This module addresses the background and capabilities of SPTFF together with implementation strategies.
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What will you be able to do after attending this course?
- Explain the theory of SPTFF and how it differs from conventional TFF processes.
- Explain the applications of SPTFF and where it is appropriate to place in a process.
- Explain the optimization of SPTFF and how it differs from conventional TFF optimization.
- Carry out SPTFF in the laboratory and analyse the data.
Course Program
Day 1: 9:00 – 17:30
- Welcome and course introduction
- SPTFF principles
- Value and use
- Process development and implementation
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- Case studies
- Practical session
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MFTFF Module: Two-pump Microfiltration
Microporous and “open UF” TFF operations are commonly used for primary or secondary clarification of products, such as lysates and cell cultures. These highly permeable membranes can lead to very high initial conversions but poor fluxes over the length of the process.
This module investigates why this phenomenon occurs and provides novel system design strategies to prevent it.
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What will you be able to do after attending this course?
- Explain the theory of TFF-MF and how it differs from TFF-UF processes.
- Explain the challenges in optimising TFF-MF processes and how to mitigate them.
- Operate and optimize a TFF-MF process in the laboratory and analyse the data.
Course Program
Day 1: 9:00 – 17:30
- Welcome and course introduction
- MFTFF theory
- Operating parameter optimization
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- Scale-up
- Practical session
- Assessment
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Which of your challenges does this course address?
- Awareness of new process demands on the TFF unit operation
- Fully understanding the theoretical and practical implications of advances in TFF
- Implementation and development of TFF techniques and processes
Hands-on Practical Section
The laboratory exercise takes place using a realistic feed stream and typical process conditions. Depending on the advanced modules chosen, the laboratory sessions may include:
- Optimising TFF microfiltration and highly permeable ultrafiltration processes using permeate control
- Successfully operating very high-concentration TFF processes
- Implementation of single-pass TFF
What will you receive?
- A copy of the course materials.
- Protocol and results of laboratory sessions.
- A certificate of attendance.
Who should attend?
This course is designed for technically capable engineers, scientists and managers within the biopharmaceutical industry who are responsible for designing, implementing and troubleshooting large-scale TFF systems for possible or current clinical manufacture.
Enrollment Limited to 8 Participants
Due to the hands-on nature of this course, enrollment is limited to 8. We recommend early booking to ensure a place.
Ordering Information
Catalogue Numbers:
- HVTFF Module: TRTFF04HV
- SPTFF Module: TRTFF04SP
- MFTFF Module: TRTFF04MF
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